Sequences from confirmation amplicon


Confirmation sequences for line 011F01 and hit At3g56580 are shown.
Line ID 011F01
Locus At3g56580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20962177
Primers used for PCR 49kb - 8474, show primer details
   
Sequence Name 49-K113379-0022-011-F01C-49kb
GenBank Accession LN506906 [GenBank]
Sequencing primer gene specific primer, 49kb: 5'-TGATTGAGCTGCTCTTTCTGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113379-0022-011-F01C-49kb
TTATTTTGTTTCATTTTTCTTGTTGGATTTAGATCCTTGGATCAAATTTGCTCAAATGTT
GTGGTAAAGTCTGTGCCTTTGATTGTATTTCGGATATTTTCTGTGTGTTTTACTCTGATA
TTGATTAGGATTTGTGTATTTCGTATTTTTTTTTTCCATTGAAGCGTTTTCTGTGATCTG
GTTTTTGATCGATCTTATTCCTCTTGATTAACCGAACGAAAGATGTTTTGATTTCTGTAG
ATCTCTGGCATTGTACTTTCTTCATTGTTCTTAATTCGATTTTCTCTCCATTGCTGGATC
GCCTGATGGCTCATTTAGAGTTTTTTTTTCTAGCTCAAATGTGGTTGAACTATAGAGTTT
CTGAGTTCATTATTTGGTCATTTTTTAATTGAGTTGGCTTGATTAAAGAAAAATTTATTT
GAGTTCATTGTTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAAATTTCC
   
Sequence Name 11-K113377-0022-011-F01C-8409
GenBank Accession LN491193 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K113377-0022-011-F01C-8409
GCATTACATTGAAATATTCACAATGAACTCAAATAAATTCTTCTTTAATCAAGCCAACTC
AATTAAAAAATGACCAAATAATGAACTCAGAAACTCTATAGTTCAACCACATTTGAGCTA
GAAAAAAAAACTCTAAATGAGCCATCAGGCGATCCAGCAATGGAGAGAAAATCGAATTAA
GAACAATGAAGAAAGTACAATGCAAGAGATCTACAGAAATCAAAACATCTTTCGTTCGGT
TAATCAAGAGGAATAAGATCGATCAAAAACCAGATCACAGAAAACGCTTCAATGGAAAAA
AAAAATACGAAATACACAAATCCTAATCAATATCAGAGTAAAACACACAGAAAATATCCG
AAATACAATCAAAGGCACAGACTTTACCACAACATTTGAGCAAATTTGATCCAAGGATCT
AAATCCAACAGGAAAAATGAAACAAAATAAAATGATAAGATTCAAGAGAACAATTTACTT
GGTTAATCAAAAA

Last Updated on 06.12.2018 16:11