Sequences from confirmation amplicon


Confirmation sequences for line 076C04 and hit At3g57200 are shown.
Line ID 076C04
Locus At3g57200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21172230
Primers used for PCR 06lu - 8474, show primer details
   
Sequence Name 31-K113947-0022-076-C04C-06lu
GenBank Accession LN500248 [GenBank]
Sequencing primer gene specific primer, 06lu: 5'-ACAAACTCAAACCACGCTCTTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K113947-0022-076-C04C-06lu
GGTTTCACAGCTATCATCTCTTACGGTNATAATCCAAGAGCTAAGAGAAGCGGGTGTTTT
TAGCTCTGTGGTAATCGCAGCTCACAAGTCTCCTTCGAAGAACTCTTCAACTGCTGATTC
GACATCAGGCATTACCCGAGAATCATCTCAGGAAACTGGTAAGAGGAGGGTGTTGGAATT
CCATCTTGATGTTGACGGTGAATCCCAAGCATCAGCGGTACCACCACAATCGCCTCCAGG
CTTGGAAGCAACCCAGATGGAACTATAATATGAAACGTGTTTACAAAGGGTGGGATTTTT
TAGAATGATTTATGAACAGTTTTGAACCTGAGCTCTGGTGTACATTTAGGAAAGTTTTCG
TTTAGGTTATACACACAGATGATTCTACAGGTGAATACAAAATGTAATGAGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 03-K113946-0022-076-C04C-8409
GenBank Accession LN487571 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K113946-0022-076-C04C-8409
ATTTACTCATTACATTTTGTATTCACCTGTAGAATCATCTGTGTGTATAACCTAAACGAA
AACTTTCCTAAATGTACACCAGAGCTCAGGTTCAAAACTGTTCATAAATCATTCTAAAAA
ATCCCACCCTTTGTAAACACGTTTCATATTATAGTTCCATCTGGGTTGCTTCCAAGCCTG
GAGGCGATTGTGGTGGTACCGCTGATGCTTGGGATTCACCGTCAACATCAAGATGGAATT
CCAACACCCTCCTCTTACCAGTTTCCTGAGATGATTCTCGGGTAATGCCTGATGTCGAAT
CAGCAGTTGAAGAGTTCTTCGAAGGAGACTTGTGAGCTGCGATTACCACAGAGCTAAAAA
CACCCGCTTCTCTTAGCTCTTGGATTATAACCTGTAAGAGATGATAGCTTGTGGAAAACC
AAACACTGATCGTTCATGTCAAATAAAGAGCGTGGTAGNAGTTTG

Last Updated on 06.12.2018 16:11