Sequences from confirmation amplicon


Confirmation sequences for line 153A10 and hit At3g60800 are shown.
Line ID 153A10
Locus At3g60800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22469204
Primers used for PCR NK21 - 3144, show primer details
   
Sequence Name 20-K102590-0022-153-A10C-3144
GenBank Accession LN495163 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102590-0022-153-A10C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTATAAA
CCTAACAGAACTTGAATACGTTCCACGCCATTGTTGTACCAGATCTATGCATAGTCAAAA
GCAACGATCGAAAGAAAACCTTTCTGGATCTTCCTTCCGGAGTAAAAGGAAAGACGCGTA
GTCAAGGCCAAGGAAGAAATGAAATAAGTGTTTCTGGGGAGATTTCGAAGAGGTCTTAGG
GTTTCTTTAAATCTGGAAACAAAATCAAAACAAGGGGTTTAAGGAATAATAATATGAGAG
TGCTAAATTGTAAGATGCAGAGACCCCATGAAGGAGGAATGATGCGGAGCTTGAGGAATG
AGACTTTTTCTCCTTCTTGGATTCGTTTTCCTCTTTTGGAGCTAACAAGAAATTTCAGAC
GAAACACTTTTAACTTTAGATTTGTAATTTAATGACTTTTAGTCAATCAATCTTTCAGAA
GCCCAAACTCAGACTGTGTAAATAATATGTATTTTTTACTACTAGCGTCAGAGAATGTTA
CATTTCGGTAATTTCCCCGGTTGATTCAAAGCAACCCAAGCATCTTGCGGTGAACATACT
TTACATCTTGAAATTCCAAATTT
   
Sequence Name 20-K102591-0022-153-A10C-NK21
GenBank Accession LN495164 [GenBank]
Sequencing primer gene specific primer, NK21: 5'-CCAAGGTTAAAACAAAAATTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102591-0022-153-A10C-NK21
GTTCACCGCAGATGCTTGGGTTGCTTTGAATAACCGGGGAAATTACCGAAATGTAACATT
TTCTGACGCTAGTAGTAAAAAATACATATTATTTACACAGTTTGAGTTTGGGCTTCTGAA
AGATTGATTGACTAAAAGTCATTAAATTACAAATCTAAAGTTAAAAGTGTTTCGTCTGAA
ATTTCTTGTTAGCTCCAAAAGAGGAAAACGAATCCAAGAAGGAGAAAAAGTCTCATTCCT
CAAGCTCCGCATCATTCCTCCTTCATGGGGTCTCTGCATCTTACAATTTAGCACTCTCAT
ATTATTATTCCTTAAACCCCTTGTTTTGATTTTGTTTCCAGATTTAAAGAAACCCTAAGA
CCTCTTCGAAATCTCCCCAGAAACACTTATTTCATTTCTTCCTTGGCCTTGACTACGCGT
CTTTCCTTTTACTCCGGAAGGAAGATCCAGAAAGGTTTTCTTTCGATCGTTGCTTTTGAC
TATGCATAGATCTGGTACAACAATGGCGTGGAACGTATTCAAGTTCTGTTAGGTTTATAC
ACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCT
CCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTG
CGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTG
GAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGT
Primers used for PCR CB39 - 8474, show primer details
   
Sequence Name 03-K104736-0022-153-A10C-CB39
GenBank Accession LN487477 [GenBank]
Sequencing primer gene specific primer, CB39: 5'-AAAAACCACTGAGAAATAGCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K104736-0022-153-A10C-CB39
AGATAAAACCATCCTTGTCTAATTCACAAAACTTCAATCTAAAACAGAACAAGACTTCAC
ATCTAAAGCAATAAAAAAGGACCAAACTTTATTGACAGAATTGAGTAAAGCACAAAGCTT
TAGTATTTTGAATTGGGGAAGATGATTGACTTACGAGGAAATGGAAGAGGATTAAGATAG
TGAGAGCAGCAAGAGAATCAAGGCCACCTTGAGAGAGTGCAGGACCATAATTAGTCAAAA
CGACAGCGTAATAAGTAACACCGACAACACCAAGAACCAAAAGGATCATGATCGACATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 19-K104737-0022-153-A10C-8409
GenBank Accession LN494749 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104737-0022-153-A10C-8409
GTCGATCATGATCCTTTTGGTTCTTGGTGTTGTCGGTGTTACTCATTACGCTGTCGTTTT
GACTAATTATGGTCCTGCACTCTCTCAAGGTGGCCTTGATTCTCTTGCTGCTCTCACTAT
CTTAATCCTCTTCCATTTCCTCGTAAGTCAATCATCTTCCCCAATTCAAAATACTAAAGC
TTTGTGCTTTACTCAATTCTGTCAATAAAGTTTGGTCCTTTTTTATTGCTTTAGATGTGA
AGTCTTGTTCTGTTTTAGATTGAAGTTTTGTGAATTTAGACAAGGATGTGTTTTTATCTT
GGTGCAGCTGGCAATGCTTTTGTGGAGCTATTTCTCAGTGGTTTT

Last Updated on 06.12.2018 16:11