Sequences from confirmation amplicon


Confirmation sequences for line 632B04 and hit At3g62980 are shown.
Line ID 632B04
Locus At3g62980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23275109
Primers used for PCR KG41 - 8474, show primer details
   
Sequence Name 75-K038032-022-632-B04C-KG41
GenBank Accession LN513193 [GenBank]
Sequencing primer gene specific primer, KG41: 5'-TTTGTTTCATCAAGAAGACTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K038032-022-632-B04C-KG41
ACTTTTGTTTGTATTAAACTTATTGGTGTGTGATTTGTATATTCATTATACTACATAAAG
TCTCAATTATAGCAAATTCATGTACTTGTTATTTGATCTGGGCATATAGGTTTTGCTGCG
TTGTTCCTGCTGATGTTACTCTGGTCTTTGTGAGCTTTTTTTTGGTTTGGTCAAGATTTT
GCGTATCTAAAAGATATATGTTTTAATCACGTTTCTGAAGATTCTTATTATAGCCACTCT
CTTTTTCTTTCTGGTGGGTCTTATTTAACGGTGGTGTATCTATTTATTCCTGGATTATTC
GCTCTCAGTGAATCTCCTGAGGTGGGAAATCTAATCAATTTGGCTTTGTGTGTGTTACTT
TTCTTTTTGTAGGGGACTTTCAATTTTCTTTTTTTACCTTGTTGGGAAACTTAGATCTGT
TTTCGGATTCCCCTTCTTTACTCGCTTTAGCTCTGTTTTAGCATCTCTCACATTGCTTCC
ACGTGATAAATTTAGTAATTTGTTATTGTTCTATGCTTAGATAGTTTCCCAAAGTGATCT
TTCTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACT
   
Sequence Name 19-K038032-022-632-B04C-8409
GenBank Accession LN494674 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K038032-022-632-B04C-8409
CCATTTACAATTGAATATATCCAGAAAGAATCACTTTGGGAAACTATCTAAGCATAGAAC
AATAACAAATTACTAAATTTATCACGTGGAAGCAATGTGAGAGATGCTAAAACAGAGCTA
AAGCGAGTAAAGAAGGGGAATCCGAAAACAGATCTAAGTTTCCCAACAAGGTAAAAAAAG
AAAATTGAAAGTCCCCTACAAAAAGAAAAGTAACACACACAAAGCCAAATTGATTAGATT
TCCCACCTCAGGAGATTCACTGAGAGCGAATAATCCAGGAATAAATAGATACACCACCGT
TAAATAAGACCCACCAGAAAGAAAAAGAGAGTGGCTATAATAAGAATCTTCAGAAACGTG
ATTAAAACATATATCTTTTAGATACGCAAAATCTTGACCAAACCAAAAAAAAGCTCACAA
AGACCAGAGTAACATCAGCAGGAACAACGCAGCAAAACCTATATGCCCAGATCAAATAAC
AAGTACATGAATTTGCTATAATTGAGACTTTATGTAGTATAATGAATATACAAATCACAC
ACCAATAAGTTTAATACAAACAAAAGCTTCTTCCTAGAGACACAAGGGAGTCTTCT
Primers used for PCR KG40 - 8474, show primer details
   
Sequence Name 18-K038032-022-632-B04C-ORil
GenBank Accession LN494218 [GenBank]
Sequencing primer gene specific primer, ORil: unknown
Sequence (A. th genome BLAST matches underlined) 
>18-K038032-022-632-B04C-ORil
GCCATTTCAATTGAATATATCCTGACAGGGTACCCGGGGATCTTTTCATGTAGATTGCCT
TCGGTTTCTCCATAATGACAATTATGCTCATGTATTGTAGGCAATGTTTATGTTCTCGAA
GTTGTATAATGTGGGTCACTCGATGCTAATTATGTGATTTCTTATGCAGGAATCTGAAAG
AGCTTGACTTACGAGAGAGTGATGTTGACGACGTTAGTGGCCACTGGCTTAGCCATTTCC
CAGATACATACACTTCTTTGGTATCACTCAATATATCTTGCTTAGCATCTGAGGTCAGTT
TCTCTGCTCTGGAAAGGCTGGTGACTAGGTGTCCCAATCTCAAGTCTCTCAAGCTTAACC
GAGCTGTTCCACTTGAAAAATTGGCTACTTTACTTCAAAGAGCACCTCAATTGGAGGAAT
TGGGCACTGGTGGGTACACTGCAGAAGTGCGACCAGATGTTTACTCTGGTTTATCTGTAG
CGCTCTCTGGGTGCAAGGAATTGAGGTGCTTATCTGGATTTTGGGATGCTGTTCCTGCCT
ATCTTCCAGCAGTTTATTCGGTTTGCAGTCGGCTTACAACTTTGAATCTGAGTTATGCAA
CAGTCCAGAGCTATGATCTTGTCAAGCTTCTTTGTCAATGCCCTAAACTGCAGCGCCTCT
GGGTGA
   
Sequence Name 74-K038032-022-632-B04C-KG40
GenBank Accession LN513023 [GenBank]
Sequencing primer gene specific primer, KG40: 5'-TGTATGTTAAAATGGACTCACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K038032-022-632-B04C-KG40
GGCATTGACAAGAAGCTTGACAGATCATAGCTCTGGACTGTTGCATAACTCAGATTCAAA
GTTGTAAGCCGACTGCAAACCGAATAAACTGCTGGAAGATAGGCAGGAACAGCATCCCAA
AATCCAGATAAGCACCTCAATTCCTTGCACCCAGAGAGCGCTACAGATAAACCAGAGTAA
ACATCTGGTCGCACTTCTGCAGTGTACCCACCAGTGCCCAATTCCTCCAATTGAGGTGCT
CTTTGAAGTAAAGTAGCCAATTTTTCAAGTGGAACAGCTCGGTTAAGCTTGAGAGACTTG
AGATTGGGACACCTAGTCACCAGCCTTTCCAGAGCAGAGAAACTGACCTCAGATGCTAAG
CAAGATATATTGAGTGATACCAAAGAAGTGTATGTATCTGGGAAATGGCTAAGCCAGTGG
CCACTAACGTCGTCAACATCACTCTCTCGTAAGTCAAGCTCTTTCAGATTCCTGCATAAG
AAATCACATAATTAGCATCGAGTGACCCACATTATACAACTTCGAGAACATAAACATTGC
CTACAATACATGAGCATAATTGTCATTATGGAGAAACCGAAGGCAATCTACATGAAAAGA
TCCCCGGGTACCCTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATC

Last Updated on 06.12.2018 16:11