Sequences from confirmation amplicon


Confirmation sequences for line 142F06 and hit At4g00020 are shown.
Line ID 142F06
Locus At4g00020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10363
Primers used for PCR HP10 - 3144, show primer details
   
Sequence Name 05-K024389-022-142-F06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K024389-022-142-F06C-8409
TCCTTGAAGCATCAAATAATAATTCTACCTCCTTTGAGTCACATGA
   
Sequence Name 06-K024389-022-142-F06C-HP10
GenBank Accession LN488636 [GenBank]
Sequencing primer gene specific primer, HP10: 5'-AAGAACTACAGACTTCCCTAGCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K024389-022-142-F06C-hp10
ATTGGGATTTCCCCAGGCATCGCTTCTTCCCGTGCTATAAGCTTCGAGCATCCTTGAAGC
ATCAAATAATAATTCAATCTCCTTTGAGTCACATGACAAACACGATTCGACAAAAAAAGG
AAGAAACGAAGAATCACCTTGGAGCAAGAGATCGGCCATAGAAGGTAGAGGAGCTGTCGA
TTCGAGCGCCTTGGTTGGCGTAGAATCCGAGACGGACTGAAGAATCCGAGACACTGATAG
TTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGA
AATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCC
CTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCT
TCTTTTTCCACGATGCTCCTCGTGGGTGGGGG
Primers used for PCR FL18 - 8474, show primer details
   
Sequence Name 04-K034547-022-142-F06C-FL18
GenBank Accession LN487818 [GenBank]
Sequencing primer gene specific primer, FL18: 5'-CGTTCTGCATGTTTAGAAATTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K034547-022-142-F06C-Fl18
GCATGTACTTTCTGTGATACTTGGCTATTGTTGCAATGTAATTTTCATTGAAGTCAGTCA
CCAATGACCATTGAACAGATGTTTTAAAATGTCCACGTAAGCAATTGTTGTTTGGAACGA
TTTTCGGTTAGCATTCGTGCCTTTTTTACCTCAATAAAAGGCCGTGGCATTAACTCACTT
GTCTTGAGACGTTATGTCGTTAAAACCAAGCCCAAAGTTAATTTCGGCCCAAATCGTTTT
CAACTTTCCCGCCGTCTTTTAACTTTTTAGATTTTTGGTGATGGTTTTGGGGGGAAAGAA
AACAGAAAATATAAAAATAAAATTTCCGCACTCCCGACTCTTGGTGTGGTAGGCAATTCT
GAATCGCGAGGTTTAGTTCTCCGGCTGAATCAGAGAACGACGAGTCGGAAAAATTCGAAA
CTCTAGAACTGAATAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 03-K034547-022-142-F06C-8409
GenBank Accession LN487381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K034547-022-142-F06C-8409
GGACTGAGCCATTTAAATTGAATATTTATTCAGTTCTAGAGTTTCGAATTTTTCCGACTC
GTCGTTCTCTGATTCAGCCGGAGAACTAAACCTCGCGATTCAGAATTGCCTACCACACCA
AGAGTCGGGAGTGCGGAAATTTTATTTTTATATTTTCTGTTTTCTTTCCCCCCAAAACCA
TCACCAAAAATCTAAAAAGTTAAAAGACGGCGGGAAAGTTGAAAACGATTTGGGCCGAAA
TTAACTTTGGGCTTGGTTTTAACGACATAACGTCTCAAGACAAGTGAGTTAATGCCACGG
CCTTTTATTGAGGTAAAAAAGGCACGAATGCTAACCGAAAATCGTTCCAAACAACAATTG
CTTACGTGGACATTTTAAAACATCTGTTCAATGGTCATTGGTGACTGACTTCAATGAAAA
TTACATTGCAACAATAGCCAAGTATCACAGAAAGTTACATTGCAACAATTTCCACGTCAA
ATTTCTAAA

Last Updated on 06.12.2018 16:11