Sequences from confirmation amplicon


Confirmation sequences for line 537H02 and hit At4g00040 are shown.
Line ID 537H02
Locus At4g00040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14800
Primers used for PCR JL07 - 8474, show primer details
   
Sequence Name 48-K032522-022-537-H02C-8409
GenBank Accession LN506417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K032522-022-537-H02C-8409
CATTACTTTCTTTGACAACTTTTTTTCTTTGAGCACTTGTGCAAAGATAAGCGTGTTTGT
GTGTCTTGGTGATGTTAGGCAAAAGCACAACTGTCAAGACACGCTACACAGTCATGTCAC
GGGAGACGCTGCACAAATACCCTGAACTAGCAACCGAGGGTTCCCCAACCATCAAACAGA
GGCTTGAGATTGCAAACGATGCGGTTGTGCAGATGGCATATGAAGCGAGCTTGGTTTGCA
TCAAGGAATGGGGAAGGGCAGTGGAAGATATCACTCATCTTGTCTACGTTTCCTCCAGTG
AGTTCCGTTTGCCCGGAGGTGATCTTTACCTCTCGGCACAGCTGGGCCTTAGCAACGAGG
TTCAGAGAGTGATGCTGTATTTTCTTGGATGCTATG
   
Sequence Name 48-K032522-022-537-H02C-JL07
GenBank Accession LN506418 [GenBank]
Sequencing primer gene specific primer, JL07: 5'-CATAGCATCCAAGAAAATACAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K032522-022-537-H02C-JL07
TGCCGAGAGGTAAGATCACCTCCGGGCAAACGGAACTCACTGGAGGAAACGTAGACAAGA
TGAGTGATATCTTCCACTGCCCTTCCCCATTCCTTGATGCAAACCAAGCTCGCTTCATAT
GCCATCTGCACAACCGCATCGTTTGCAATCTCAAGCCTCTGTTTGATGGTTGGGGAACCC
TCGGTTGCTAGTTCAGGGTATTTGTGCAGCGTCTCCCGTGACATGACTGTGTAGCGTGTC
TTGACAGTTGTGCTTTTGCCTAACATCACCAAGACACACAAACACGCTTATCTTTGCACA
AGTGCTCAAAGAAAAAAAGTTGTCAAAGAAAGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR FP74 - 8474, show primer details
   
Sequence Name 60-K035339-022-537-H02C-8409
GenBank Accession LN510183 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K035339-022-537-H02C-8409
GCCATTTACTCATTGCATATCGCATTTGATTTCACGGAGATACTCCTCCACGAGATTCTC
CTGGGAAACAACATTGCTCGGCAAGGCCTTACCGAGAGCAAGCACTGTCGCCTTCCCCTG
ATAAGCAACACGCTTTTGTTTCTCTACCCTTGCGGACACCAACATCTTTGTGAGTTCACA
ATGAAATAAGAGACAAAGGATTAGTGTTGGCCTTTTAAGTCATGGATTGTGAAAATAACA
TTAAAAAAGGGCATAGAAAATGTGGGTGGTGTGTTTGTAACAAACACAGACAAAAACCCT
AAATAAACTCAAGGATGACACTTTGAATGATCCTTCAAATTCCTCTTCCTCCTCCTCCCT
ATATTCCTGCATCATCTATTTGAGGCCCAATGATAAAGGAGAGAGACAGAAATGGTGTAT
AACTTATACAAGAAGCAAAAAGCATCACA
   
Sequence Name 60-K035339-022-537-H02C-FP74
GenBank Accession LN510184 [GenBank]
Sequencing primer gene specific primer, FP74: 5'-AATTTGTGATGCTTTTTGCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K035339-022-537-H02C-FP74
CATTTCTGTNCTCTCCTTTATCATTGGGCCTCCATAGATGATGCAGGAATATAGGGAGGA
GGAGGAAGAGGAATTTGAAGGATCATTCAAAGTGTCATCCTTGAGTTTATTTAGGGTTTT
TGTCTGTGTTTGTTACAAACACACCACCCACATTTTCTATGCCCTTTTTTAATGTTATTT
TCACAATCCATGACTTAAAAGGCCAACACTAATCCTTTGTCTCTTATTTCATTGTGAACT
CACAAAGATGTTGGTGTCCGCAAGGGTAGAGAAACAAAAGCGTGTTGCTTATCAGGGGAA
GGCGACAGTGCTTGCTCTCGGTAAGGCCTTGCCGAGCAATGTTGTTTCCCAGGAGAATCT
CGTGGAGGAGTATCTCCGTGAAATCAAATGCGATATGCAATGAGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11