Sequences from confirmation amplicon


Confirmation sequences for line 843C09 and hit At4g00335 are shown.
Line ID 843C09
Locus At4g00335 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:146986
Primers used for PCR DR55 - 8474, show primer details
   
Sequence Name 02-K035539-022-843-C09C-8409
GenBank Accession LN486936 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K035539-022-843-C09C-8409
AAGCCATTTACAATTGAATATATAGATAGATAGTCAGTAGTGCAAAGCAACACAAATGCA
AGATGGTGAAAAGGATTACTGACATAATAGTAAACAGGTGTTCCAACAAGATGTGACTTC
CTCGAAGAACTACAGCAACCTCCCATTTTCACCAGCTATAGTAATAATTAAGCAAGAGTA
GCGAACAACAGTCCTTGAAGCATTTTACAGATGCACTGCCACTGCAGGAAAAGAGTGAAC
TGAACAACGATTACTTGAATGCAACAAGATTAATCAAAAGTACTCTTAAGAAACCCCAAT
AAAAAAAAAGTGATTATTACAGTGTTAGTAAGCAAAATGACAAAAGAAAAGTTGGTCTGA
TGGCAAAGTCATCCATAAGAAACACAAGGATTGGGGAATGGATGAATAGTGAGAAGAACA
TTACGAAACAAGATTAATCAAAGCAGACTTGAGAAATCCCAATAACAAGTGATTATTACA
GTGTTAATAAGCAAAATGACAAAAGAAAAAGTTGATCTGATGCCAAAGTTATCCATAAGA
AACACAAAGGTATGG
   
Sequence Name 17-K035539-022-843-C09C-DR55
GenBank Accession LN493765 [GenBank]
Sequencing primer gene specific primer, DR55: 5'-CATTCCCATACCTTTGTGTTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K035539-022-843-C09C-DR55
CTTACTAACACTGTAATAATCACTTTTTTTTTATTGGGGTTTCTTAAGAGTACTTTTGAT
TAATCTTGTTGCATTCAAGTAATCGTTGTTCAGTTCACTCTTTTCCTGCAGTGGCAGTGC
ATCTGTAAAATGCTTCAAGGACTGTTGTTCGCTACTCTTGCTTAATTATTACTATAGCTG
GTGAAAATGGGAGGTTGCTGTAGTTCTTCGAGGAAGTCACATCTTGTTGGAACACCTGTT
TACTATTATGTCAGTAATCCTTTTCACCATCTTGCATTTGTGTTGCTTTGCACTACTGAC
TATCTATCTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11