Sequences from confirmation amplicon


Confirmation sequences for line 701G05 and hit At4g00600 are shown.
Line ID 701G05
Locus At4g00600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:255369
Primers used for PCR 26qt - 8474, show primer details
   
Sequence Name 51-K115779-0022-701-G05C-26qt
GenBank Accession LN507602 [GenBank]
Sequencing primer gene specific primer, 26qt: 5'-GGTCTCTTGTTTATCCTTGGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K115779-0022-701-G05C-26qt
ATGATGAGCTGGGGTAGACCATTTTGAAGCCCGGATTGATCTCCGCCCAACCTGTTGCTG
ATTAATTTCTGAAGTTTTGGTATTTTGTGGAGAAAAGTTTCAGAATCTGTAACATAGGAG
TCATGGGACACACCAAAATGACTTCCAACATCAATCGTAATGACTTCATCCTTAAGCTAT
CTTCCAAGACTTACATTACGTTAAAAGATACATTTGATAAAGCAAAAATGCTTTTGCCAA
TCACCACAGTTGCTTCATGGTAGTTCTACTCCCAAAATGAACAAAAGCTCACACACAAAC
TTATAAAGATCCAAATAATTATCTAGCAAAGCCGCAAGATAATCAGGGAACTCACAATCT
GGATAAGGTAACCTCTAAAATCAAAGAACAAGAACCGAAATAATATTATTTCTGACTTAA
CGCTAAGCAAAAATCATTGGCTTCAAAAAACCAAAGTAAGATTTGTTTATTTAAAAAAAG
GCGAGCAATCATCTTTGAAGTCACTGGAAGTTGTGAATTCTCTTGGCTGATGTTAAAGTG
TTGGATAGAACTACACACTTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGT
   
Sequence Name 03-K115778-0022-701-G05C-8409
GenBank Accession LN487624 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115778-0022-701-G05C-8409
AAGTGTGTAGTTCTATCCAACACTTTAACATCAGCCAAGAGAATTCACAACTTCCAGTGA
CTTCAAAGATGATTGCTCGCCTTTTTTTAATAAACAAATCTTACTTTGGTTTTTTGAAGC
CAATGATTTTTGCTTAGCGTTAAGTCAGAAATAATATTATTTCGGTTCTTGTTCTTTGAT
TTTAGAGGTTACCTTATCCAGATTGTGAGTTCCCTGATTATCTTGCGGCTTTGCTAGATA
ATTATTTGGATCTTTATAAGTTTGTGTGTGAGCTTTTGTTCATTTTGGGAGTAGAACTAC
CATGAAGCAACTGTGGTGATTGGCAAAAGCATTTTTGCTTTATCAAATGTATCTTTTAAC
GTAATGTAAGTCTTGGAAGATAGCTTAAGGATGAAGTCATTACGATTGATGTTGGAAGTC
ATTTTGGTGTGTCCCATGACTCCTATGTTACAGATTCTGAAACTTTTCTCCACAAAATAC
CAAAACTTCAGAAATTAATCAGCAACAGGTTGGGCGGAGATCAATCCGGGCTTCAAAATG
GTCTACCCCAGCCTCACTCATCTGTCTCCTCGTCCTGAGAATCGAAATCTGTCCNAGGAT
AAACAAGAGAC

Last Updated on 06.12.2018 16:11