Sequences from confirmation amplicon


Confirmation sequences for line 002E12 and hit At4g02120 are shown.
Line ID 002E12
Locus At4g02120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:942299
Primers used for PCR 52op - 8474, show primer details
   
Sequence Name 54-K114782-0022-002-E12C-52op
GenBank Accession LN508567 [GenBank]
Sequencing primer gene specific primer, 52op: 5'-ACCAATGTACTTTCCAACCATAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114782-0022-002-E12C-52op
ATGTGTCAGTGTAAATACTCACATGATTTGTCAAGTTATCAAAAGTTTCAGCCATCTTAT
TCCAGCTATCCAGATCAGGTGCTGTCGCAACGCTGAAAGAGATGAGTTTATTGAACTTGG
TGTTACACTGAAAGTGATATCGGCTAACTTATGAAGACCATATAAGAAATGAGACTAACT
TAGTTAGATTCAGTTGTTTGAGAATCGAGTGATGAGCGTTTTGATTCTGCAGCAAGCCAA
GAGCATACATGATTCTATTTCCTTTATGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 54-K114769-0022-002-E12C-8409
GenBank Accession LN508566 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114769-0022-002-E12C-8409
CTTTCATAAGTTAGCCGAATATCACTTTTCAGCGTAACACCAAGTTTCAATAAACTCATC
TCTTTCAGCGTTGCGACAGCACCTGATCTGGATAGCTGGAATAAGATGGCTGAAACTTTT
GATAACTTGACAAATCATGTGAGTATTTACACTGACACATTTTCTTTTACAAGCCTTGAC
TTTTTCTTATTTCTGTTATGCACAGGTCCAAATTGCTATGGT

Last Updated on 06.12.2018 16:11