Sequences from confirmation amplicon


Confirmation sequences for line 105D09 and hit At4g02510 are shown.
Line ID 105D09
Locus At4g02510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1106428
Primers used for PCR 15hz - 3144, show primer details
   
Sequence Name 27-K112562-0022-105-D09C-15hz
GenBank Accession LN498459 [GenBank]
Sequencing primer gene specific primer, 15hz: 5'-ATAGTCGATGATGCAGAAGAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112562-0022-105-D09C-15hz
TCTGCTATTGAAGCTGTGAATGTAGATGTGGCAAAGCCAGGGGTTGTTGTTGTTGGAGAC
GTAGAGGTGAGTGAGGTGCTGGAAACTGATGGCAACATCCCTGATGTGCATAATAAGTTT
GATCCAATTGGCCAAGGTGAAGGTGGTGAAGTTGAACTTGAATCCGATAAAGCCACAGAG
GAGGGAGGAGGAAAGTTGGTTAGTGAAGGAGACTCTATGGTTGACTCCTCTGTGGTGGAC
TCTGTTGATGCTGATATCAATGTTGCAGAGCCAGGGGTTGTGGTTGTCGGGGCGGCTAAG
GAGGCTGTAATTAAGGAAGATGATAAAGATGATGAGGTTGACAAGACCATCTCAAATATT
GAGGAACCTGATGACCTGACTGCTGCATATGATGGAAATTTATCAAACACTGATAGTTTA
AACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATG
AACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTA
CGTCAGAGAAGATATCACATCAATCCA
   
Sequence Name 03-K112561-0022-105-D09C-3144
GenBank Accession LN487554 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112561-0022-105-D09C-3144
CAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATAAATTTCCATCATAT
GCAGCAGTCAGGTCATCAGGTTCCTCAATATTTGAGATGGTCTTGTCAACCTCATCATCT
TTATCATCTTCCTTAATTACAGCCTCCTTAGCCGCCCCGACAACCACAACCCCTGGCTCT
GCAACATTGATATCAGCATCAACAGAGTCCACCACAGAGGAGTCAACCATAGAGTCTCCT
TCACTAACCAACTTTCCTCCTCCCTCCTCTGTGGCTTTATCGGATTCAAGTTCAACTTCA
CCACCTTCACCTTGGCCAATTGGATCAAACTTATTATGCACATCAGGGATGTTGCCATCA
GTTTCCAGCACCTCACTCACCTCTACGTCTCCNACAACAACAACCCCTGGCTTTGCCACA
TCTACATTCACAGCTTCAATAGCAGATGAGTCCACCACAAAGTCTCCTTTGTTGTTTAAC
TTAACAACTTCATCTG

Last Updated on 06.12.2018 16:11