Sequences from confirmation amplicon


Confirmation sequences for line 293C04 and hit At4g04080 are shown.
Line ID 293C04
Locus At4g04080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1963749
Primers used for PCR CQ46 - 8474, show primer details
   
Sequence Name 07-K105747-0022-293-C04C-CQ46
GenBank Accession LN489271 [GenBank]
Sequencing primer gene specific primer, CQ46: 5'-AGAAAGCCCTAGAATTTCACAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105747-0022-293-C04C-CQ46
TGTTAGGCAACACTAAAAGAGCCTTTTTAGGGCTCGCTTCTCAAAATCCCACACCGTTCC
CTGTCGTGTCTCGATTATACCATCCAAATGTCATCGACCATTATGACAATCCCCGTAACG
TTGGATCATTTGATAAGAATGATCCTAACGTTGGTACGGGATTGGTCGGAGCTCCTCAAT
GTGGAGATGTTATGAAGCTTCAGGTTAAGTTTGATGGTTCTGGTCAAATCATTGATGCTA
AATTCAAGACCTTTGGTTGTGGTTCTGCCATAGCAGCTTCTTCCGTCGGTATGGTACCTT
CTTATGTGATATTGTGATCGATTTGTTTCTCTAATCCTGAAATCTTTAAATTAATTGATT
GATATTGTGATTAATTGATTACCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 23-K105748-0022-293-C04C-8409
GenBank Accession LN496555 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K105748-0022-293-C04C-8409
CATTTACATTGAATTAGGTAATCAATTAATCACAATATCAATCAATTAATTTAAAGATTT
CAGGATTAGAGAAACAAATCGATCACAATATCACATAAGAAGGTACCATACCGACGGAAG
AAGCTGCTATGGCAGAACCACAACCAAAGGTCTTGAATTTAGCATCAATGATTTGACCAG
AACCATCAAACTTAACCTGAAGCTTCATAACATCTCCACATTGAGGAGCTCCGACCAATC
CCGTACCAACGTTAGGATCATTCTTATCAAATGATCCAACGTTACGGGGATTGTCATAAT
GGTCGATGACATTTGGATGGTATAATCGAGACACGACAGGGAACGGTGTGGGATTTTGAG
AAGCGAGCCCTAAAAAGGCTCTTTTAGTTGTTTGCCTTAACATGATCGATCGCTTCTCTC
TATGGAGCTTGTTGTGAAATTCTGGGGCTTTC

Last Updated on 06.12.2018 16:11