Sequences from confirmation amplicon


Confirmation sequences for line 322A03 and hit At4g04720 are shown.
Line ID 322A03
Locus At4g04720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2395512
Primers used for PCR DL42 - 8474, show primer details
   
Sequence Name 19-K018491-022-322-A03C-DL42
GenBank Accession LN494556 [GenBank]
Sequencing primer gene specific primer, DL42: 5'-ACATATTTTAGAGGCTCAAAGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K018491-022-322-A03C-DL42
AACAAGATAGTCAGTCCATTGTATAGGACCTCTAGGTCTAAATAGACTCTAGTGAATGTT
GAAGTCTGATCTTGTTCTTGTCTTTGAGTTTATGGTCGTCTAACTAGTAATGCTTTACTA
ATATGTAGCCATCCTTATGGTTTATAGAACATCCTTGGATCAAAGGGGGAGAAGCACCAG
ACAAGCCTATTGATAGCGCTGTGTTATCCCGCATGAAGCAATTCCGAGCAATGAACAAGC
TTAAGAAGCTAGCTCTAAAGGTAAAGAAGTGGCTGAAATTTTTTATGAAATTATCTTTCT
TTATTACTAATTTTGCATTTCAATTATCCACTTGCAATCTCTCTATGCTCCATATTGCAA
TGCCTCATATCTACTGGTGCCTGAAATTTTGGTTGTAATTTGTGTATTCTGTTTTCAGGT
TATCGCGGAGAGTCTATCAAAAGAGGAGATTAAAGGTCTTAAAACCATGTTTGCGAATAT
AGATACCGACAAAAGCGGGAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATTGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTT
   
Sequence Name 20-K018491-022-322-A03C-8409
GenBank Accession LN495011 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K018491-022-322-A03C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTTCCCGCTTTTGTCGGTATC
TATATTCGCAAACATGGTTTTAAGACCTTTAATCTCCTCTTCTGATAGACTCTCCGCGAT
AACCTGAAAACAGAATACACAAATTACAACCAAAATTTCAGGCACCAGTAGATATGAGGC
ATTGCAATATGGAGCATAGAGAGATTGCAAGTGGATAATTGAAATGCAAAATTAGTAATA
AAGAAAGATAATTTCATAAAAAATTTCAGCCACTTCTTTACCTTTAGAGCTAGCTTCTTA
AGCTTGTTCATTGCTCGGAATTGCTTCATGCGGGATAACACAGCGCTATCAATAGGCTTG
TCTGGTGCTTCTCCCCCTTTGATCCAAGGATGTTCTATAAACCATAAGGATGGCTACATA
TTAGTAAAGCATTACTAGTTAGACGACCATAAACTCAAAGACAAGAACAAGATCAGACTT
CAACATTCACTAGAGTCTATTTAGACCTAGAGGTCCTATACAATGGACTGACTATCTTGT
TCTGGCTTTGAGCCTCTAANATATGTAGCCATCCTTATGGTTTATAGAACATCCTTGGAT
CAAAGGGGGAGAAGCACCAGACAAGCCTATTGATAGCGCTGTGTTATCCCGCAT

Last Updated on 06.12.2018 16:11