Sequences from confirmation amplicon


Confirmation sequences for line 337G08 and hit At4g06540 are shown.
Line ID 337G08
Locus At4g06540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:3376292
Primers used for PCR 18qz - 8474, show primer details
   
Sequence Name 42-K115714-0022-337-G08C-18qz
GenBank Accession LN504583 [GenBank]
Sequencing primer gene specific primer, 18qz: 5'-CAATTCCAATCTTAACAGGCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K115714-0022-337-G08C-18qz
GTCAGCTAGAGTAAACTAAGTTGTAGAACTTGTATTCCATGATTCCTAGCCTTTTAGCTG
TAGAGAGAGGCATAAGGCTAACAGATGCTCCCAAATCACAAAAGGCACCTATCGAATGTC
CATGGTCCTAGAGAGCATGGAAGAGTGAATGAACCGGGATCATCAAGCTTTTCTTAAGTG
ACTTTTTTCTTGTAAGTTTCTTGAGTGATGACTCTACTAATCAACTCTTGATACTGATCC
TTCCTGAGTTTTTCTCTTTTGAAATCTTTTTCAGAGTGGTGAGTATTCAGCAAAGGTGTG
TGCTCTATAATCCTGATGAATTGCAGGGTGGTCGTGCAGCAATTTGAGTTGAATCCCCCC
CCCCTTCTTGAATCAAACTGTCCTCAGTATTGGGAGTCTGATGACTTGGTGGAGACTCAT
CTTCTTGGTGGATGGTGATAGTATTTGTAGTAGCATAGTCCTTAGGATTTTGAACTGCTT
TTCCTGGAAGTTGCTGAGGATGCTTCGGAGTAAACTCTCCACATACTTGATTTTGGAATT
CAGAGCTTCAAACTTGACAGTTAGATCGTTGTAACAACAATCAATCTTATTGTGTAATTC
AGCTATCTTCTTGTCAAAAGCCATAGCTCCAGTAGCTTGTCCTTGAATGATTTGTTGAAT
GAGTTGCTTAATTTCAGGATCTTGAGCTGTTGGGGATTGTTGTGGTTGTTGAGTGAACCC
TGGTGGTGGATTCTGCTGGTTCTAGCATCCATTAAATTGTTGTTTAGGGATATAACCTTG
GTTGTACTGAACAAATGGCTTAGACTGATTCTGTTGTGGTGGATACACTTGATCTTGTCC
GGGAAATCTACTTGGATCAGCAATG
   
Sequence Name 02-K115713-0022-337-G08C-8409
GenBank Accession LN487171 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115713-0022-337-G08C-8409
TATCCACCAAACAGAATCAGTCTAAGCCATTTGTTCAGTACAACCAAGGTTATATCCCTA
AACAACAATTTAATGGATGCTAGAACCAGCAGAATCCACCACCAGGGTTCACTCAACAAC
CACAACAATCCCCAACAGCTCAAGATCCTGAAATTAAGCAACTCATTCAACAAATCATTC
AAGGACAAGCTACTGGAGCTATGGCTTTTGACAAGAAGATAGCTGAATTACACAATAAGA
TTGATTGTTGTTACAACGATCTAACTGTCAAGTTTGAAGCTCTGAATTCCAAAATCAAGT
ATGTGGAGAGTCTACTCCGAAGCATCCTCAGCAACTTCCAGGAAAAGCAGTTCAAAATCC
TAAGGACTATGCTACTACAAATACTATCACCATCCACCAAGAAGATGAGTCTCCACCAAG
TCATCAGACTCCCAATACTGAGGACAGTATGATTCAAGAAGGGGGGGGGGATTCAACTCA
AATTGCTGCACGACCACCCTGCAATTCATCAGGATTATAGAGCACACACCTTTGCTGAAT
ACTCACCACTCTGAAAAAGATTTCAAAAGAGAAAAACTCAGGAAGGATCAGTATCAAGAG
TTGATTAGTAGAGTCATCACTCAAGAAACTTACAAGAAAAAAGTCACTTAAGAAAAGCTT
GATGATCCCGGTTCATTCACTCTTCCATGCTCTCTAGGACCATGGACATTCGATAGGTGC
CTTTTGTGATTTGGGAGCATCTGTTAGCCTTATGCCTCTCTCTACAGCTAAAAGGCTAGG
AATCATGGAATACAAGTTCTACAACTTAGTTTTACTCTTAGCTGACGGTTCTGTTGCACA
TCCCCATGATCTAATAGAAAATTTGCCTG

Last Updated on 06.12.2018 16:11