Sequences from confirmation amplicon


Confirmation sequences for line 478D06 and hit At4g12720 are shown.
Line ID 478D06
Locus At4g12720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7489878
Primers used for PCR 21zb - 8474, show primer details
   
Sequence Name 15-K123633-0022-478-D06C-21zb
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 21zb: 5'-TATGAAGGGTGTCAGCGTATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K123633-0022-478-D06C-21zb
GTTGTTGGTGATTGCATAAGAAAGTAGTTCTTACATCTTACAGATCCATCAAGTGTTTAC
TGATAATCTGTAATGATACCAAGAATGAAACTAAGAGAATGGTGGATATGAAGTTTTGAT
TGTTGTTGTCTTTTGCTTCTAACTAAAACATTCCAATGCCACAGGGCGAGGATATATGGA
CTGGAGTAGCTAGGGAAGTGGAAGAAGAAACTGGAGTAAGTATCTGGCTTTCAAAAATTA
AGCATTTCAATCATAAGGATCTCTTACTTGACATTCTAATCTAAAATTTGTGTTCTTCTC
TTGGCTTTTCTCTTCAGATTATTGCAGATTTTGTCGAAGTACTGGCTTTCAGGTAACTTA
AATTTTATTGCTGTAGACACATTGATCTGGCTTAAACGCATTTGTTCCTCATTGTTCAAC
TCTTTACACTCTCAGGCAAAGCCACAAAGCCATCTTAAAAAAGAAAACAGATATGTTTTT
CCTGTGTGTCTTAAGTCCGCGCTCTTACGATATTACTGAACAAAAATCTGAGATCTTGCA
AGCTAAGGTAAACCTTTCTAACTTTCAGTGTTGATGAGCCAAGAAAATGTTAAAAGTTAC
TGACATATATTTCAAATTTAACCGAAACTGGTATATTGTATTACAGTGGATGCCGATCCA
AGAGTATGTAGACCAACCATGGAACAAGAAGAACGAGATGTTCAAGTTCATGGCTAACAT
TTGCCAAAAGAAGTGTGAGGAAGAATACTTGGGATTCGCCATTGTGCCAACTACCACATC
ATCTGGTAAAGAGAGCTTTATCTACTGCAATGCGGATCATGCCAAGCGCCTTAAAGTATC
GCGTGACCAAGCCTCTGCTTCTCTCTGAAAACATTCTCATCTGATTCTGTTTGATCTCGG
CATGTGTGTGTCCAGTGTGTACAAAACAGGAAACTT
   
Sequence Name 07-K123632-0022-478-D06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K123632-0022-478-D06C-8409
AATTTGTAGTAAATGGTCAAGAGACGATACAGCTGAGAACTTAAAACCGGTTACGAGAAG
ATATTTTGGAAGAATAAACCAAGTGTATGGTAACACTCTTAAGCCTCATATCTTTAAAAA
GCACGTAAGCGTTGGGAGAATCAAACCATCACTGGAAATAGACTAGAAGTCGATGGCAAG
TTTTACAGTGGCTCATAGAGATGGTATAAACTTAAATATTTAGACGCAATTTTGTAGAAT
CGGAATAATATAGAAGAAAGTTTCCTGTTTTGTACACACTGGACACACACATGCCGAGAT
CAAACAGAATCAGATGAGAATGTTTTCAGAGAGAAGCAGAGGCTTGGTCACGCGATACTT
TAAGGCGCTTGGCATGATCCGCATTGCAGTAGATAAAGCTCTCTTTACCAGATGATGTGG
TAGTTGGCACAATGGCGAATCCCAAGTATTCTTCCTCACACTTCTTTTGGCAAATGTTAG
CCATGAACTTGAACATCTCGTTCTTCTTGTTCCATGGTTGGTCTACATACTCTTGGATCG
GCATCCACTGTAATACAATATACCAGTTTCGGTTAAATTTGAAATATATGTCAGTAACTT
TTAACATTTTCTTGGCTCATCAACACTGAAAGTTAGAAAGGTTTACCTTAGCTTGCAAGA
TCTCAGATTTTTGTTCAGTAATATCGTAAGAGCGCGGACTTAAGACACACAGGAAAAACA
TATCTGTTTTCTTTTTTAAGATGGCTTTGTGGCTTTGCCTGAGAGTGTAAAGAGTTGAAC
AATGAGGAACAAATGCGTTTAAGCCAGATCAATGTGTCTACAGCAATAAAATTTAAGTTA
CCTGAAAGCCAGTACTTCGACAAAATCTGCAATAATCTGA

Last Updated on 06.12.2018 16:11