Sequences from confirmation amplicon


Confirmation sequences for line 344G06 and hit At4g15090 are shown.
Line ID 344G06
Locus At4g15090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8614947
Primers used for PCR 12hg - 8474, show primer details
   
Sequence Name 57-K112176-0022-344-G06C-12hg
GenBank Accession LN509462 [GenBank]
Sequencing primer gene specific primer, 12hg: 5'-CCATTGATTTCATATTGCTTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K112176-0022-344-G06C-12hg
GCGCTGGTCCTTATGAAGTTTGGTTGTGACATTTTTCAGGTGCTAAGGTGGATTATCTAG
TAGAAAGATTTCAGTTTCCAATTTCATGGAAGCCCATAGTGGGGTAGTTGCTTTTGAGTG
TGTTAGTGAATCCATATGTGGCTAAGGTTAGGATGGATTTGCAAGAGAATCTGGTTAGTG
ATGCAGGGGATGACCATATGGTTGACATTGTAGTTGAACCGCATAGTAACAGAGATATAG
GAATTGTTGATGAGTTCAATATAGGTGGAGATGTTGGATTTAGTGGTGATCTGGCCATGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCG
   
Sequence Name 41-K112175-0022-344-G06C-8409
GenBank Accession LN504128 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112175-0022-344-G06C-8409
ATCATGGCCAGATCACCACTAAATCCAACATCTCCACCTATATTGAACTCATCAACAATT
CCTATATCTCTGTTACTATGCGGTTCAACTACAATGTCAACCATATGGTCATCCCCTGCA
TCACTAACCAGATTCTCTTGCAAATCCATCCTAACCTTAGCCACATATGGATTCACTAAC
ACACTCAAAAGCAACTACCCCACTATGGGCTTCCATGAAATTGGAAACTGAAATCTTTCT
ACTAGATAATCCACCTTAGCACCTGAAAAATGTCACAACCAAACTTCATAAGAACCAGCG
CGACATCTTCTACCTAAAAACATCAAACTTGAAACACACAAAGCAA

Last Updated on 06.12.2018 16:11