Sequences from confirmation amplicon


Confirmation sequences for line 511D04 and hit At4g17560 are shown.
Line ID 511D04
Locus At4g17560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9780065
Primers used for PCR 06sv - 8474, show primer details
   
Sequence Name 71-K116641-0022-511-D04C-06sv
GenBank Accession LN512620 [GenBank]
Sequencing primer gene specific primer, 06sv: 5'-CTCTACATTTCCTTCCGTATCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K116641-0022-511-D04C-06sv
CTTCTTCTGCTCTAACGATAAACTCATTTCTCACCCTAGTATCGAGTGAGAACCCAAATT
TCGAGCCGGGATGATTCAGAGAAAGCCTAGAAACTGATATACGAGAATCCATCGATGGTG
CACACGATAGAAACGAAGAAAACCCTAAATTCTTCGATGATTGCGTAGGGTTTCTGGGTA
TCATATGCAATGCCTAAAAATCCCATAGAAACAAATAATCAACAATAATGAATTAAGTGA
AAATAGAGATTTCATAAGCTTTCGTATATTTAAACCTGAGGAAGACGATGAAAGCTCATC
GCCATAGCTGCAGCAAACTCTCAGTTACTCGGAGAAAATGGAGGGAAGACAGTGGAAAAG
AGCCTTATCTGCTTTTCGCTTCCTATTTTGGCACACGTATGCCATCGCACGTGTGAATTT
TCTTTATGGGCCGTTTGTTATTGTGGGCCTTTAGTATATCAATATTAATATAAATAGATA
GTACTATGATTTATAAAAATATCAATAAAAGAGTCGGCTAATGACACAGTCTTATTATGA
TTACATAAACCTATAATTTGGGCCCACCACCACGTCGGTCGTGTGAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 58-K116640-0022-511-D04C-8409
GenBank Accession LN509808 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K116640-0022-511-D04C-8409
CATTCCACGACCGACGTGGTGGTGGGCCCAAATTATAGGTTTATGTAATCATAATAAGAC
TGTGTCATTAGCCGACTCTTTTATTGATATTTTTATAAATCATAGTACTATCTATTTATA
TTAATATTGATATACTAAAGGCCCACAATAACAAACGGCCCATAAAGAAAATTCACACGT
GCGATGGCATACGTGTGCCAAAATAGGAAGCGAAAAGCAGATAAGGCTCTTTTCCACTGT
CTTCCCTCCATTTTCTCCGAGTAACTGAGAGTTTGCTGCAGCTATGGCGATGAGCTTTCA
TCGTCTTCCTCAGGTTTAAATATACGAAAGCTTATGAAATCTCTATTTTCACTTAATTCA
TTATTGTTGATTATTTGTTTCTATGGGATTTTTAGGCATTGCATATGATACCCAGAAACC
CTACGCAATCATCGAAGAATTTAGGGTTTTCTTCGTTTCTATCGTGTGCACCATCGATGG
ATTCTCGTATATCAGTTTCTAGGCTTTCTCTGAATCATCCCGGCTCGAAATTTGGGTTCT
CACTCGATACTAGGGTGAGAAATGAGTTTATCGTTAGAGCAGAAGAAGGTAACACAGAAG
CTGAATCTGAAGAATTTGTTGCTGAAATCGCTGATACGG

Last Updated on 06.12.2018 16:11