Sequences from confirmation amplicon


Confirmation sequences for line 611A10 and hit At4g18610 are shown.
Line ID 611A10
Locus At4g18610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10250530
Primers used for PCR IS60 - 8474, show primer details
   
Sequence Name 55-K023621-022-611-A10C-8409
GenBank Accession LN508680 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K023621-022-611-A10C-8409
ATATTTCCCGGATATAATGCATAGTTTTGAAATTCTTAAATAAATTATTTTGGTTTCGCA
TGAAAATATGAAAGGAGAGAAATTTATTATTGTCACTTATATATATATACATCGTAATCA
TTTTTTCGTGAATAATTCTCTCTCCCATTCCATTATTTCTCAGTATCTCTCTTTCTTTCC
CTTACTTTATTGTTGCTTTTAAACCTTCAATTTGCTCATAAACCAAATATATAATATCAA
AACAAACAAACAAAAAATCAGAATTCCCCTAATAATGTCTTCGGATCGTCACACACCGAC
GAAAGATCCACCGGATCATCCGTCTTCTTCCTCCAACCACCACAAGCAACCACTTCCTCC
TCAACCGCAGCAACCACTCAGCCGCTATGAATCGCAGAAACGCCGCGACTGGAACACGTT
CGTCCAATACCTAAAATCACAAAATCCACCGTTGATGATGTCTCAATTCGACTACACGCA
CGTGCTAAGTTTCCTAAGGTACTTAGATCAGTTTGGTAAGACCAAAGTACATCATCAAGC
TTGTGTCTTCTTCGGACAACCGGATCCACCAGGTCCGTGCACGTGTCCTCTCAAACAAGC
TTGGGGAAGCCTAGATGCTTTGATCGGA
   
Sequence Name 56-K023621-022-611-A10C-IS60
GenBank Accession LN508982 [GenBank]
Sequencing primer gene specific primer, IS60: 5'-ACCTCGTTTTTAGTCTTCCTCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K023621-022-611-A10C-IS60
TTCTTCCTGTACGGAATCCCACGAGCCTTGGCTTGAGATTCTCTCACTTCCCTCAAGTGA
ACCCGGATCGACCCGTTTGCAAACGGGTTAGTATCAGGTGACCCGCCACCGTGTTCCTCG
TAAGCAGCTCTTAGCCGTCCGATCAAAGCATCTAGGCTTCCCCAAGCTTGTTTGAGAGGA
CACGTGCACGGACCTGGTGGATCCGGTTGTCCGAAGAAGACACAAGCTTGATGATGTACT
TTGGTCTTACCAAACTGATCTAAGTACCTTAGGAAACTTAGCACGTGCGTGTAGTCGAAT
TGAGACATCATCAACGGTGGATTTTGTGATTTTAGGTATTGGACGAACGTGTTCCAGTCG
CGGCGTTTCTGCGATTCATAGCGGCTGAGTGGTTGCTGCGGTTGAGGAGGAAGTGGTTGC
TTGTGGTGGTTGGAGGAAGAAGACGGATGATCCGGTGGATCTTTCGTCGGTGTGTGACGA
TCCGAAGACATTATTAGGGGAATTCTGATTTTTTGTTTGTTTGTTTTGATATTATATATT
TGGTTTATGAGCAAATTGAAGGTTTAAAAGCAACAATAAAGTAAGGGAAAGAAAGAGAGA
TACTGAGAAATAATGG

Last Updated on 06.12.2018 16:11