Sequences from confirmation amplicon


Confirmation sequences for line 354D01 and hit At4g20910 are shown.
Line ID 354D01
Locus At4g20910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11186120
Primers used for PCR NK54 - 8474, show primer details
   
Sequence Name 13-K103453-0022-354-D01C-NK54
GenBank Accession LN492003 [GenBank]
Sequencing primer gene specific primer, NK54: 5'-ACCTAATTGTTTCATTACAACGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103453-0022-354-D01C-NK54
CACTCGATCTCCGCGTTTATTGCAGTCCAAACAATGATATAGCTCTGTTCGGATCTTTAT
CTATCAACTGTTCTTCCACATAAACAATATCCATTTGTTAGAAAACCTCAATCATAGACA
GGTTCAAGAAGATCACTCAAAATTTGAAAAGCTTTTAAGAGATTTTATCACATGAGGAAG
AATCTAGAATTCCTAAATTAGGTCAAAAGAAAAATTCCCCTGTCTTGTTTTCGCGGGAAA
ATTGATTATACCTGCGCGTGTTTGGCTCTGACGTAAGGAGAATCACCAGAAGGAACTTTA
TGAACAATGTGAAACGGATCACGTCTCTCCGACGACGAAGAACAAGAATACGGTGGTCTC
CTCCTCTCCGTCATCGGCATCGCCGCCACATGATCACTTGTTCTCGCCGGCGGCGGAGTA
TAGTAAACTCCAGGTGGAGCACGACGCTCTACGCAGGGACACATTTTCTTTTTCGATTCT
CTCTGTCAATTTACTCAGATAAAGTTCGAGAAAGCTTTTCCCAGAATTCAAATTCTCGTG
TTCTTCAGAAATCGCCCTCGTGAAAAAGATCAAGAACGCAAATGCTTTGAAAACGAATCA
GTGTCGTTGAAGAAGTGAGAAAGTGATAAAAATCACAAGGACTTGTATTTAAATAAACAA
ACTTTAACGTAGGGGCAAAAAGGGTAATCTACTGATCTAATCTTTAAACTTCAATGATCT
ACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 29-K103454-0022-354-D01C-8409
GenBank Accession LN499248 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103454-0022-354-D01C-8409
TAGATCATTGAAGTTAAAGATTAGATCAGTAGATTACCCTTTTTGCCCCTACGTTAAAGT
TTGTTTATTTAAATACAAGTCCTTGTGATTTTTATCACTTTCTCACTTCTTCAACGACAC
TGATTCGTTTTCAAAGCATTTGCGTTCTTGATCTTTTTCACGAGGGCGATTTCTGAAGAA
CACGAGAATTTGAATTCTGGGAAAAGCTTTCTCGAACTTTATCTGAGTAAATTGACAGAG
AGAATCGAAAAAGAAAATGTGTCCCTGCGTAGAGCGTCGTGCTCCACCTGGAGTTTACTA
TACTCCGCCGCCGGCGAGAACAAGTGATCATGTGGCGGCGATGCCGATGACGGAGAGGAG
GAGACCACCGTATTCTTGTTCTTCGTCGTCGGAGAGACGTGATCCGTTTCACATTGTTCA
TAAAGTTCCTTCTGGTGATTCTCCTTACGTCAGAGCCAAACACGCGCAGGTATAATCAAT
TTTCCCGCGAAAACAAGACAGGGGAATTTTTCTTTTGACCTAATTTAGGAATTCTAGATT
CTTCCTCATGTGATAAAATCTCTTAAAAGCTTTTCAAATTTTGAGTGATCTTCTTGAACC
TGTCTATGATTGAGGTTTTCTAACAAATGGATATTGTTTATGTGGAAGAACAGTTGATAG
ATAAAGATCCGAACAGAGCTATATCATTGTTTTGGACTGCAATAAACGCTGGAGATCGAG
TTGATAGTGCTTTGAAGGATATGGCCGTTGT
Primers used for PCR 94wr - 8474, show primer details
   
Sequence Name 24-K118742-0022-354-D01C-94wr
GenBank Accession LN497201 [GenBank]
Sequencing primer gene specific primer, 94wr: 5'-CGTTTATCAAAAGCAATCTGTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118742-0022-354-D01C-94wr
TCTGTAGGTATTGAACCATGGAAGAAGATCAAGCCTGTGAATTTGGGGAAAAAGTTCTGA
GCTTGTTCCACCCCAAGCTCCTGATTGTCTCAACACCAAACTACGAGTTCAACACAATTC
TCCAGCGGTCTACACCAGAAACCCAAGAAGAAAACAACTCGGAGCCACAGCTTCCAAAGT
TCAGGAACCATGACCACAAATTCGAGTGGACAAGAGAACAGTTCAATCAATGGGCATCAA
AGCTCGGCAAACGCCATAACTACAGCGTCGAGTTTAGCGGTGTTGGTGGGTCTGGTGAAG
TAGAACCCGGATTTGCTTCTCAGATAGCTATTTTTAGACGGGAAGCTTCATCTGTTGAGA
ATGTTGCAGAAAGCTCAATGCAGCCTTATAAAGTCATCTGGGAGTGGAAGAAAGAAGATG
TAGAAAAGAAAAAGACTGATCTTTGACTAGAGTAGTATCTACCAGAGAATATATATATTG
CAAGCAATGTGATGACGTGTTCATCTATAAAACTCAAAGCTCGTTTAGTTCTTTTGCCAT
GAGCTTTTGCAGATTAACCATAAAATCGAACCTTTATGTTTACAAGATCCTTTTGTCTCC
AAGTCTACTGGTCCGTAAATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 08-K118741-0022-354-D01C-8409
GenBank Accession LN489942 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118741-0022-354-D01C-8409
GGATCTTGTAACATAAAGGTTCGATTTTATGGTTAATCTGCAACAGCTCATGGCAAAAGA
ACTAAACGAGCTTTGAGTTTTATAGATGAACACGTCATCACATTGCTTGCAATATATATA
TTCTCTGGTAGATACTACTCTAGTCAAAGATCAGTCTTTTTCTTTTCTACATCTTCTTTC
TTCCACTCCCAGATGACTTTATAAGGCTGCATTGAGCTTTCTGCAACATTCTCAACAGAT
GAAGCTTCCCGTCTAAAAATAGCTATCTGAGAAGCAAATCCGGGTTCTACTTCACCAGAC
CCACCAACACCGCTAAACTCGACGCTGTAGTTATGGCGTTTGCCGAGCTTTGATGCCCAT
TGATTGAACTGTTCTCTTGTCCACTCGAATTTGTGGTCATGGTTCCTGAACTTTGGAAGC
TGTGGCTCCGAGTTGTTTTCTTCTTGGGTTTCTGGTGTAGACCGCTGGAGAATTGTGTTG
AACTCGTAGTTTGGTGTTGAGACAATCAGGAGCTTGGGGTGGAACAAGCTCAGAACTTTT
TCCCCAAATTCACAGGCTTGATCTTCTTCCATGTGTTCAATAACCTACAGATTAGGAACA
GATTATAGATTTAACGCCGAAACCAGACAGATTGCTTGGGATAAACG

Last Updated on 06.12.2018 16:11