Sequences from confirmation amplicon


Confirmation sequences for line 875C10 and hit At4g21100 are shown.
Line ID 875C10
Locus At4g21100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11259433
Primers used for PCR 83ra - 8474, show primer details
   
Sequence Name 41-K116132-0022-875-C10C-83ra
GenBank Accession LN504209 [GenBank]
Sequencing primer gene specific primer, 83ra: 5'-TTCAGTTGTATAAGTGGATGCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116132-0022-875-C10C-83ra
ATCTCTTTATTCGATCTACAAGGTAAAACGTTTTTGGAATACGAAATTCGTTTTTCCGTC
TTTATATGAATACATGCCAAATCTTAGATTGTTATGTTCACAAGAACTCACCGGACTCTT
TTGATTATCTGTGTTTTGTTTTAAAGCACGAGGAAGGTGCGATCGAGGAGAGAGCTCGGG
ACTATAATGCAAACTGGATGACGGCAGTTGAGATACTCAATGATGACATCTACCTCGGTA
CTGATAATTGCTTCAACATATTCACCGTAAAGAAGAACAACGAAGGTGCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATT
   
Sequence Name 16-K116131-0022-875-C10C-8409
GenBank Accession LN493576 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116131-0022-875-C10C-8409
TTCAACCGCCGTCATCCAGTTTGCATTATAGTCCCGAGCTCTCTCCTCGATCGCACCTTC
CTCGTGCTTTAAAACAAACACAGATAATCAAAAGAGTCCGGTGAGTTCTTGTGAACATAA
CAATCTAAGATTTGGCATGTATTCATATAAAGACGGAAAAACAATTTCGTATCCAAAACG
TTTTACCTTGTAGATCAATAAAGAGATTGATTTCATGAGATCACCAACAGCGATGAAGTC
TCCACGGGTCTGCACGTAGAGAGCTAGTATGTGACCGTGATGTCCACATTCAGATTGCAG
CTCGCGAGTGCCATCATCCCGCAGCATCCA

Last Updated on 06.12.2018 16:11