Sequences from confirmation amplicon


Confirmation sequences for line 665D10 and hit At4g21540 are shown.
Line ID 665D10
Locus At4g21540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11460588
Primers used for PCR JV12 - 8474, show primer details
   
Sequence Name 74-K032930-022-665-D10C-JV12
GenBank Accession LN513003 [GenBank]
Sequencing primer gene specific primer, JV12: 5'-TCAAGAGGAAGTAAAGCCATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K032930-022-665-D10C-JV12
CTTGAATTCAGGTTTCTATTTCTATTATCTTCTTCCATTCTACTGAGGGAAATTAACAAT
CGGATTACTTTTGATATTTGCTTCAGAAACCAAGTATCAGTTGCATGCAAAGGAAATTGT
TAGGTCCATGGATGTATCAAAATACGATGGTATTGTTTGTGTTAGCGGTGACGGTATCCT
TGTTGAGGTAAGTAAGAAATTTTCTTTATGTTGTGGAAGTATCATTTTCTCTATGTATAT
TTTATTGGATATCTTAATTTGGAAGTGATGATGCGTGTCTTTCGTGCTGTTCTCACATGA
TACTTACTAAAGCAATTTAGAATTGATATCTTAGACAAAGTAAGAGGAATTGCTTTTGGG
AGTATCTGTAGAGGAAGCAATCTCTTGTTTAATTATATGTTTACTGTTTTGCTTATGTTT
GACTCTGGGAAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 74-K032930-022-665-D10C-8409
GenBank Accession LN513002 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K032930-022-665-D10C-8409
TTACATTGAATATATCCTGATTTCCCAGAGTCAAACATAAGCAAAACAGTAAACATATAA
TTAAACAAGAGATTGCTTCCTCTACAGATACTCCCAAAAGCAATTCCTCTTACTTTGTCT
AAGATATCAATTCTAAATTGCTTTAGTAAGTATCATGTGAGAACAGCACGAAAGACACGC
ATCATCACTTCCAAATTAAGATATCCAATAAAATATACATAGAGAAAATGATACTTCCAC
AACATAAAGAAAATTTCTTACTTACCTCAACAAGGATACCGTCACCGCTAACACAAACAA
TACCATCGTATTTTGATACATCCATGGACCTAACAATTTCCTTTGCATGCAACTGATACT
TGGTTTCTGAAGCAAATATCAAAAGTAATCCGATTGTTAATTTCCCTCAGTAGAATTGGA
AGAAGATAATAGAAATTAGAAACCTTGAATCTCAAGTTGAATATTTGCATCTTCAAACAA
TGGCTTT

Last Updated on 06.12.2018 16:11