Sequences from confirmation amplicon


Confirmation sequences for line 515A05 and hit At4g21600 are shown.
Line ID 515A05
Locus At4g21600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11486233
Primers used for PCR 70qa - 8474, show primer details
   
Sequence Name 47-K115481-0022-515-A05C-70qa
GenBank Accession LN506292 [GenBank]
Sequencing primer gene specific primer, 70qa: 5'-TCTTGGATACGGCTATAACTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K115481-0022-515-A05C-70qa
GAAGGCTTGAATTGTAGTATGTTTCTAGAGCAGAGTCAATTATCATGTTGTCCCAGACCT
TCAAGTTAAGATCGTTCCACTTCCGTGTCAGGAAAGATACTTTTTAGCATCAATATGACT
AAAAAACAAAGCACTTACATGGTGCAAATTTGATTTGTTATGGTACCAGTTGACTATTAT
TGTGTTACCACCTAGATCTCCAAGAAAACCAGTATGCAAGGGCTGCGAAAATCATAATCC
ACATAAAGATTAGCCATACTCTTGTGTTGCTACTATACACTAATCAAACCTCCAGAGTCC
AGAGTAGATAGAGTAGTAGCCTCAAATGCATATAACTCTGACAGCATTTGTTTGTAATAT
TATGATTCGACTGAATTTGCAAAAAGGACTAACCTGATGAACATCTCCCATATNATGTGA
CAAGAACAAGAGAGCCATAAAACCAAAATCTACATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGT
   
Sequence Name 19-K115480-0022-515-A05C-8409
GenBank Accession LN494893 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115480-0022-515-A05C-8409
ACATTATATGGGAGATGTTCATCAGGTTAGTCCTTTTTGCAAATTCAGTCGAATCATAAT
ATTACAAACAAATGCTGTCAGAGTTATATGCATTTGAGGCTACTACTCTATCTACTCTGG
ACTCTGGAGGTTTGATTAGTGTATAGTAGCAACACAAGAGTATGGCTAATCTTTATGTGG
ATTATGATTTTCGCAGCCCTTGCATACTGGTTTTCTTGGAGATCTAGGTGGTAACACAAT
AATAGTCAACTGGTACCATAACAAATCAAATTTGCACCATGTAAGTGCTTTGTTTTTTAG
TCATATTGATGCTAAAAAGTATCTTTCCTGACACGGAAGTGGAACGATCTTAACTTGAAG
GTCTGGGACAACATGATAATTGACTCTGCTCTAGAAACATACTACAATTCAAGCCTTCCA
CACATGATTCAAGCTCTTCAAGCCAAACTCAAGGTACAATCTTGCCTTCTCTAATCAGCT
TCAGCAAGTTAT

Last Updated on 06.12.2018 16:11