Sequences from confirmation amplicon


Confirmation sequences for line 756D01 and hit At4g21680 are shown.
Line ID 756D01
Locus At4g21680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11517805
Primers used for PCR NA53 - 8474, show primer details
   
Sequence Name 10-K101562-0022-756-D01C-8409
GenBank Accession LN490590 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101562-0022-756-D01C-8409
GCCTTTACATTGAATATATCCTTGAAGCCATTTGCGCGCTATGTATGGCTTCAATATCTC
TTGGGAACTATGTAAGCAGTTTGTTAGTTTCCATTGTCATGAAGATCTCTACAACAGATG
ATGTGCATGGCTGGATTCCTGAAAATCTCAACAAAGGACACTTGGAGAGATTCTACTTCC
TTTTAGCTGGTCTAACCGCTGCTGATTTCGTGGTTTACTTGATTTGTGCCAAATGGTACA
AGTATATTAAATCTGAAGCAAGTTTCTCTGAGTCCGTAACTGAAGAGGAGGAAGTCTGAG
TCAGGAGCTAGAGAGTTTTGTGTTTTATTTTAATGCTGTGAAGCTTTTTACAGTAATTTG
TTTAAAGCTGAGCTTGTTCTGTGATATAAACTGGTCTTATTGTGTTTTTATTTTTGGGTT
ATACTCTTGTGCAAAGTTTAGTTGATGTTTCTCAATAAAGGATAAGTTGATTCCACAGTT
CCAACACATAGTTTATGTAACTTTTCTCATAACCTTTGAATCAGTCAAAACCTAAACACT
TACTAGTAGAACAGTAAAAATTTACAATATTAAATCAAGCTCTTAATGTTGTAGTGAAAA
CAAGGACTGTTAACTATGGAAACTGCAGAAAAAGCTTTGTGGAAACTTAA
   
Sequence Name 10-K101571-0022-756-D01C-NA53
GenBank Accession LN490591 [GenBank]
Sequencing primer gene specific primer, NA53: 5'-GTTCTCAGGCTGTTAAGTTTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101571-0022-756-D01C-NA53
TCCTTGTTTTCACTACACATTAAGAGCTTGATTTAATATTGTAAATTTTTACTGTTCTAC
TAGTAAGTGTTTAGGTTTTGACTGATTCAAAGGTTATGAGAAAAGTTACATAAACTATGT
GTTGGAACTGTGGAATCAACTTATCCTTTATTGAGAAACATCAACTAAACTTTGCACAAG
AGTATAACCCAAAAATAAAAACACAATAAGACCAGTTTATATCACAGAACAAGCTCAGCT
TTAAACAAATTACTGTAAAAAGCTTCACAGCATTAAAATAAAACACAAAACTCTCTAGCT
CCTGACTCAGACTTCCTCCTCTTCAGTTACGGACTCAGAGAAACTTGCTTCAGATTTAAT
ATACTTGTACCATTTGGCACAAATCAAGTAAACCACGAAATCAGCAGCGGTTAGACCAGC
TAAAAGGAAGTAGAATCTCTCCAAGTGTCCTTTGTTGAGATTTTCAGGAATCCAGCCATG
CACATCATCTGTTGTAGAGATCTTCATGACAATGGAAACTAACAAACTGCTTACATAGTT
CCCAAGAGATATTGAAGCCATACATAGCGCGCAAATGGCTTCAAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGAT

Last Updated on 06.12.2018 16:11