Sequences from confirmation amplicon


Confirmation sequences for line 617D02 and hit At4g24510 are shown.
Line ID 617D02
Locus At4g24510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12662010
Primers used for PCR IW50 - 8474, show primer details
   
Sequence Name 17-K033212-022-617-D02C-8409
GenBank Accession LN493753 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K033212-022-617-D02C-8409
TGGGGATTGAGTCCTATTGTCGTTTCCCGCCTTCGGTTCATACTCATTGCTGATTGTAGC
TATCAATGTGGTTGAGGCTAAAATTAAAAATGTGTCTCCCCATCTTGCATTTATTGGTAA
GTAACCAATACTCGCCAACCGAATCTATCTTTTCAATGGAAATAGCTTCACCATCATTAC
GAGCATGAGAGGTTAGTTCGGGGGTTTTCGGGTAAACCGGTTTTGTTGGGGCATGACCCG
ATACCAGCTGTCCAAGTGTTTTCATGAACGTTGATGCTGAAAACACGTCTCCAAGAATAT
GGGCCCAACTCAACCCAATACAGAGCCCACCACATTTAAACTGAGTTATCTGAAAAACGA
ATTTCAAAGTAAAAAATTTAGTTTACTCTAGCTAGCCTGATTTGGACAAAAAAAAAACAC
ACCCACACACACACACAAAATCTAACCTGATCATATATAGTGAAAAATCAATTTCACATG
TAAAAAATATTTTCTCTTCAAGTTCCACGGTTATATTAAATATTCATTTCATAATTATTA
ATTAAGCTAATGATTGGAAAGAATTTGAAACGAATGTTTGATAGTTAGTAACTAAAAAAG
AAACTAAGTTCGTGGGAAAATCAGCCGTTTTCGTTGCATGTGAACTTAAATAATCCATTA
TATTATACAAAAATTTGTGTATGTGTGTTTACCGGGAGGAAAACGAGTGGCGAGAAGGTA
   
Sequence Name 25-K033212-022-617-D02C-IW50
GenBank Accession LN497338 [GenBank]
Sequencing primer gene specific primer, IW50: 5'-TCTTGGTCCTGATCTTACCTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K033212-022-617-D02C-IW50
CATACACAAATTTTAGTATAATATAATGGATTATTTAAGTTCACATGCAACGAAAACGGC
TGATTCTCCCACGAACTTAGTTTCTTTCTTAGTTACTAACTATCAAACATTCGTTTCAAA
TTCTTTCCAATCATTAGCTTAATTAATAATTATGAAATGAATATTTAATATAACCGTGGA
ACTTGAAGAGAAAATATTTTTTACATGTGAAATTGATTCTTCACTATATATGATCAGGTT
AGATTCTGTGTGTGTGTGTGTGTGTGTTTTTTTTTTGTCCAAATCAGGCTAGCTAGAGTA
AACTAAATTTTTTACTTTGAAATTCGTTTTTCAAATAACTCATTTTAAATGTGGGGGGCT
CTGTATTGGGTTGAGTTGGGCCCATATTCTTGGAGACGTGTTTTCAGCATCAACGTTCAT
GAAAACACTTGGACAGCTGGTATCGGGTCATGCCCCAACAAAACCGGTTTACCCAAAAAC
CCCCAAACTAACCTCTCATGCTCGTAATGATGGTGAAGCTATTTCCATTGAAAAAATAGA
TTCGGTTGGCGAGTATTGGTTACTTACCAATAAATGCAAGATGGGGAGACACATTTTTAA
TTTTACCCTCAACCACATTGATAGCTACAATCAGCAATGAGTATGAACCGAAGGCGGGAA
ACGACAATAGGACTTCAATCCCCAAGGGCTTCATGTCCGGGAAATCTACGTGGATCACCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCCCCGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTA

Last Updated on 06.12.2018 16:11