Sequences from confirmation amplicon


Confirmation sequences for line 891B01 and hit At4g25370 are shown.
Line ID 891B01
Locus At4g25370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12973622
Primers used for PCR 56yp - 8474, show primer details
   
Sequence Name 14-K121650-0022-891-B01C-56yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 56yp: 5'-GTCTTAAACGTCCCTATTGCGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K121650-0022-891-B01C-56yp
TTCTGGGTTTTTCCTTTCCCACGGATATTGAAGCTTAGGGTGTTCAGTTTGAATTTTTCA
GGCAACCGGAGAATGGTTCTTCGGATAAGATCCCAAAGTGAGTCTCACTCCAAATTACGT
ATAGAATTTGTAATTGTAGAGCTTATACGTTTGAATTGTTGAGGTTTTTTTTTTAATTGT
TTGATTTTAGATGGTCGGCGAGAGCTATAAAGTCACTTGCAATGGGAGAATTGGAGGCTA
GGAAGCTCAAGTATCCAAGTACCGGGACCGAAGCCATTCTCATGGGTATTTTAGTCGAAG
GTTTGTGATTCTGAGACCTATATTTGCTTCAGCTTTGTGTTTTGCCGCGTCTTCTAGTTT
AGATTTTGATTTCTAAGGATCACTATGTTGCTTTGGATATTTATGCTTTGTCATTGTGAA
AGCTTGTATTTTAGTTTTGAGGGATTCTGTACAATAATGCTGGTGAGATTGTTTCGGCTA
AGTGTGTGCTTCAGCTCATCTGAGCTTTAACTGAATCCAAGCATTGCATAAGCAAGAGCT
TTTATGATTAGTTGTCCTGTTTCTTAATCATTAAGTTGATGCTGTATAAGTCGGTTGTGG
AATGCCTTATGTTGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATATAAATGTCCCC
   
Sequence Name 13-K121794-0022-891-B01C-8474
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K121794-0022-891-B01C-8474
TCTTTTTCTCCTATTGACCACATAAGGCATTCCACAACCGACTTATACAGCATCAACTTA
ATGATTAAGAAACAGGACAACTAATCATAAAAGCTCTTGCTTATGCAATGCTTGGATTCA
GTTAAAGCTCAGATGAGCTGAAGCACACACTTAGCCGAAACAATCTCACCAGCATTATTG
TACAGAATCCCTCAAAACTAAAATACAAGCTTTCACAATGACAAAGCATAAATATCCAAA
GCAACATAGTGATCCTTAGAAATCAAAATCTAAACTAGAAGACGCGGCAAAACACAAAGC
TGAAGCAAATATAGGTCTCAGAATCACAAACCTTCGACTAAAATACCCATGAGAATGGCT
TCGGTCCCGGTACTTGGATACTTGAGCTTCCTAGCCTCCAATTCTCCCATTGCAAGTGAC
TTTATAGCTCTCGCCGACCATCTAAAATCAAACAATTAAAAAAAAAACCTCAACAATTCA
AACGTATAACCTCTACAATTACAAATTCTATACGTAATTTGGAGTGAGACTCATTTTGGG
ATCTTATCCAAAAAACCATTCTCCGGTTGCCTGAAAAATTCAACCTTAACACCCTAAGCT
TCAATATCCGTGGTGAAAGGAAAAACCCAAAATTGATAAAGATGTAAAAGAGCTGTCAAA
TAAAAAAACTAACGCATTAGGGACGTTTAAAA

Last Updated on 06.12.2018 16:11