Sequences from confirmation amplicon


Confirmation sequences for line 174H01 and hit At4g30340 are shown.
Line ID 174H01
Locus At4g30340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14840659
Primers used for PCR SD29 - 8474, show primer details
   
Sequence Name 16-K103490-0022-174-H01C-SD29
GenBank Accession LN493398 [GenBank]
Sequencing primer gene specific primer, SD29: 5'-AGTACACCGATTTTGATTTGCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K103490-0022-174-H01C-SD29
AACACAGAACTGTCAATTTATTAGTCTTATTGAATTGTCTTCAAAGTTCGATCATTTTTT
TACGTTTTATTTTAATTTGTTTCTCCAAGAATCTGATAATGGAGGAGACGCCGAGATCAG
TCGGCGAAGCATCGACGACTAATTTCGTGGCGGCGAGACCGTCGGCGAAGACCGATGATG
CAGTGACGATGAGAGGTTGTGGTTTTGCTAATCTTGCCTTGGTTGGTATAGACAAAGAAG
AGCTTCGAGGGAGACTAGCGATGCCTGAGTATCTTCGTATAGCTATGAGAGATTGTATCA
AGAGGAAAGATTCAACAGAGATTTCTGATCATTTGTTACTTCCTGGAGGCGCCGCCGCTG
ATATGGCTCCTCACGCGCCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGC
   
Sequence Name 40-K103491-0022-174-H01C-8409
GenBank Accession LN503681 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K103491-0022-174-H01C-8409
GCCTTGGCGCGTGAGGAGCCATATCAGCGGCGGCGCCTCCAGGAAGTAACAAATGATCAG
AAATCTCTGTTGAATCTTTCCTCTTGATACAATCTCTCATAGCTATACGAAGATACTCAG
GCATCGCTAGTCTCCCTCGAAGCTCTTCTTTGTCTATACCAACCAAGGCAAGATTAGCAA
AACCACAACCTCTCATCGTCACTGCATCATCGGTCTTCGCCGACGGTCTCGCCGCCACGA
AATTAGTCGTCGATGCTTCGCCGACTGATCTCGGCGTCTCCTCCATTATCAGATTCTTGG
AGAAACAAATTAAAATAAAACGTAAAAAAATGATCGAACTTTGAAGACAATTCAATAAGA
CTAATAAATTGACAGTTCTGTGTTTTGTTTTTTAAGATTAGTTTTCGAAGACAGAGCAAA
TCA

Last Updated on 06.12.2018 16:11