Sequences from confirmation amplicon


Confirmation sequences for line 270A06 and hit At4g30935 are shown.
Line ID 270A06
Locus At4g30935 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15052493
Primers used for PCR IS21 - 8474, show primer details
   
Sequence Name 33-K017280-022-270-A06C-IS21
GenBank Accession LN500790 [GenBank]
Sequencing primer gene specific primer, IS21: 5'-AAGGAAACTGTTGTAAGTGCGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K017280-022-270-A06C-IS21
GAGAATCGTCAGGTAGAAACATCTCCTTCTCTGGCTGCATCGTCAGACTCATTGACAGTG
ACGCCATGTCTATCTTTGGATCCTGCAACTGCTTCAACCGCACAAGATTTACCACTGGTT
TCAGTTCCAACTAAACAAGAGCAAAGATCAGATTCGCCGGTGGTTAACAGATTGTCGGTT
ACTCCTGTTCCTAGGACACCGGCTCGTGATGGTTATAATTGGAGAAAATATGGACAGAAG
CAGGTTAAGAGTCCCAAGGGCTCACGGAGTTACTACAGGTGTACATACACTGAATGTTGT
GCTAAAAAAATTGAATGCTCCAATGATTCAGGCAATGTGGTAGAGATTGTTAACAAAGGT
TTACATACTCATGAACCTCCCCGGAAGACTAGCTTCTCCCCGAGAGAGATTAGAGTTACA
ACAGCTATCCGGCCTGTTTCAGAGGATGATACAGTAGTAGAAGAGCTATCAATTGTTCCT
AGCGGTTCAGATCCGTCTGCTTCTACTAAAGAATACATCTGTGAGTCGCAAACACTCGTT
GACCGGAAAAGACACTGTGAGAACGAAGCTGTGGAGGAACCAGAGCCAAAACGAAGGCAA
GTTTGTAGAATTTTGCATTTCGGTGCTTACTCTTCACTTTGAAAGATGAAATTAATAGAC
TTTCTTCTCTCTATTTTTCAGACTGAAAAAAGATAACTCACAGAGTCTACATACATACTC
TGTCCGGGAAATCTACATAATCAGCAATCTGTGGTGTTTTATCACAGTGTATGTATGGCT
TCATGTCCGGGAAATCTAC
   
Sequence Name 34-K017280-022-270-A06C-8409
GenBank Accession LN501215 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K017280-022-270-A06C-8409
TGTAGATTTCCCGGACATGAAGCCATACATACACTGTGATAAAACACCACAGATTGCTGA
TTATGTAGATTTCCCGGACAGAGTATGTATGTAGACTCTGTGAGTTATCTTTTTTCAGTC
TGAAAAATAGAGAGAAGAAAGTCTATTAATTTCATCTTTCAAAGTGAAGAGTAAGCACCG
AAATGCAAAATTCTACAAACTTGCCTTCGTTTTGGCTCTGGTTCCTCCACAGCTTCGTTC
TCACAGTGTCTTTTCCGGTCAACGAGTGTTTGCGACTCACAGATGTATTCTTTAGTAGAA
GCAGACGGATCTGAACCGCTAGGAACAATTGATAGCTCTTCTACTACTGTATCATCCTCT
GAAACAGGCCGGATAGCTGTTGTAACTCTAATCTCTCTCGGGGAGAAGCTAGTCTTCCGG
GGAGGTTCATGAGTATGTAAACCTTTGTTAACAATCTCTACCACATTGCCTGAATCATTG
GAGCATTCAATTTTTTTAGCACAACATTCAGTGTATGTACACCTGTAGTAACTCCGTGAG
CCCTTGGGACTCTTAACCTGCTTCTGTCCATATTTTCTCCAATTATAACCATCACGAGCC
GGTGTCCTAGGAACAGGAGTAACCGACAATCTGTTAACCACCGGCGAATCTGATCTTTGC
TCTTGTTTAGTTGGAACTGAAACCAGTGGTAAATCTTGTGCGGTTGAAGCAGTTGCAGGA
TCCAAAGATAGACATGGCGTCACTGTCAATGAGTCTGACGATGCAGCCAGAGAAGGAGAT
GTTTCTACCTGACGATTCTCTTCCACTTCATCAACCGGCACAATCGC

Last Updated on 06.12.2018 16:11