Sequences from confirmation amplicon


Confirmation sequences for line 268D08 and hit At4g30950 are shown.
Line ID 268D08
Locus At4g30950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15057906
Primers used for PCR ZZ42 - 8474, show primer details
   
Sequence Name 13-K020608-022-268-D08C-8409
GenBank Accession LN491839 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K020608-022-268-D08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAGATGAGCACATTCA
CAATGGTTCATCATACGGCTCCGCATATACCTTTCAAGCCTGCGGATGAGTGGAACGCGG
CTCAGGCCCAGCTGAATGGAACTGTTCATTGTGACTACCCTAGTTGGTAATGCATACCAC
TTGCTCAGATGGTTAAGTTGCTGAGAGCTATCGTTTGGTTTCATATGTACTAATATTTGT
GTGTTACGACAGGATTGAAATTCTCTGCCATGATATCAACGTTCACATCCCGCATCATAT
TAGCCCAAGAATACCGAGCTACAATCTCCGTGCAGCTCATGAGTCTATACAAGAGAACTG
GGGAAAGGTACTAACCACTTTCTTATCTCGTAAATTTGTTGTGAATAACATGACAGTAAT
TTGAACCACAACATAATAAGTGTTCCTTTGGGAAGTTAATAAGACACTAAAAGTTTCCTG
GGCTACTTCTTTTGGGGTGTTGTTGCAGTATACAAACTTGGCT
   
Sequence Name 14-K020608-022-268-D08C-ZZ42
GenBank Accession LN492308 [GenBank]
Sequencing primer gene specific primer, ZZ42: 5'-CAGTTCCATGTAGCCAAGTTTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K020608-022-268-D08C-ZZ42
CTGCAACAACACCCCAAAAGAAGTAGCCCAGGAAACTTTTAGTGTCTTATTAACTTCCCA
AAGGAACACTTATTATGTTGTGGTTCAAATTACTGTCATGTTATTCACAACAAATTTACG
AGATAAGAAAGTGGTTAGTACCTTTCCCCAGTTCTCTTGTATAGACTCATGAGCTGCACG
GAGATTGTAGCTCGGTATTCTTGGGCTAATATGATGCGGGATGTGAACGTTGATATCATG
GCAGAGAATTTCAATCCTGTCGTAACACACAAATATTAGTACATATGAAACCAAACGATA
GCTCTCAGCAACTTAACCATCTGAGCAAGTGGTATGCATTACCAACTAGGGTAGTCACAA
TGAACAGTTCCATTCAGCTGGGCCTGAGCCGCGTTCCACTCATCCGCAGGCTTGAAAGGT
ATATGCGGAGCCGTATGATGAACCATTGTGAATGTGCTCATCTACAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTTATTATAAAATGAAAGTACATTTTGATAAAACG

Last Updated on 06.12.2018 16:11