Sequences from confirmation amplicon


Confirmation sequences for line 271H10 and hit At4g31690 are shown.
Line ID 271H10
Locus At4g31690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15345237
Primers used for PCR 12zd - 8474, show primer details
   
Sequence Name 75-K123843-0022-271-H10C-12zd
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 12zd: 5'-AACTAAATAAACCGCACCAGACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K123843-0022-271-H10C-12zd
TTCTTCGTAATCAAACTAACTCATGCCTTCCTTTACTAAAACCACTTCGACTTCACGAAA
TCTCTCGGCACACTCTGCATCAGTCATCAAATTGCATTAGGACTGATATAAACATAGAAA
AAAACAAAGAAACCGCTAATCCGACAAACCACTGCATCTCTTGATATGTTTGAATGCGTC
ACGGATTGGCTAAAACAATTCAGATCTGATGAAGACTTATCAGATTCTTTTTGCACATTC
TCTTCACCTTCTTTCTCGGATATTTCTGAATCAGAAAATAAACGTTTCAAGAATAGCCTA
AGATAAATGTATAAAGGTTCATTGAATGATGATTCATACCATTTTCACCACTCTCTTCGC
CTTCTTCATGTCTGCTAGACTGTGGGTATTGGATCTCACAGCAACTAGGTCCCAAAGCAG
TGACATGGAACACCATGTCTCCTTCATGTCTAAAGACAACAAAGTCACCGATTCGAAGAT
CATGTGCCTCAACGAACTCTTTCCAACCTTCCGTGAGTCTATGACCCTCCATCTTTACTT
CCCATGTTCTCTCCGACGCATCTGTTCTCAACGTTACAGTCTTGCCTTCGTGTTTTCCCT
CGATGTGCTCAGAGAAGTAGTTCACAGGAATTTTCTGCAACAGAGAAACAGAACCATCAT
GAGACTATATGTTATGTTCAGACAGATTGAGAGAAAGAAAGATTGCGTACGAGGTTGCTC
TGGAATCCGGGAAGAAGAGGCTGGAAGAAGTGTTGGTTAGTCGGAGACAAGGGTGATGCA
TTCGCCATTCCGATCACTCTNACAAAGCCNAAAGCCCAAAAGTTTTCTCGGTGACTTAAT
GTAATAAAATACAAAGGAGAATCTTTGTAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAGAAAGAGTAATTAC
   
Sequence Name 61-K123842-0022-271-H10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K123842-0022-271-H10C-8409
CATTTCAGAATCTCCTTTGTATTTTATTACATTAAGTCACCGAGAAAACTTTTGGGCTTT
TGGCTTTGTTAGAGTGATCGGAATGGCGAATGCATCACCCTTGTCTCCGACTAACCAACA
CTTCTTCCAGCCTCTTCTTCCCGGATTCCAGAGCAACCTCGTACGCAATCTTTCTTTCTC
TCAATCTGTCTGAACATAACATATAGTCTCATGATGGTTCTGTTTCTCTGTTGCAGAAAA
TTCCTGTGAACTACTTCTCTGAGCACATCGAGGGAAAACACGAAGGCAAGACTGTAACGT
TGAGAACAGATGCGTCGGAGAGAACATGGGAAGTAAAGATGGAGGGTCATAGACTCACGG
AAGGTTGGAAAGAGTTCGTTGAGGCACATGATCTTCGAATCGGTGACTTTGTTGTCTTTA
GACATGAAGGAGACATGGTGTTCCATGTCACTGCTTTGGGACCTAGTTGCTGTGAGATCC
AATACCCACAGTCTAGCAGACATGAAGAAGGCGAAGAGAGTGGTGAAAATGGTATGAATC
ATCATTCAATGAACCTTTATACATTTATCTTAGGCTATTCTTGAAACGTTTATTTTCTGA
TTCAGAAATATCCGAGAAAGAAGGTGAAGAGAATGTGCAAAAAGAATCTGATAAGTCTTC
ATCAGATCTGAATTGTTTTAGCCAATCCGTGACGCATTCAAACATATCAAGAGATGCAGT
GGTTTGTCGGATTAGCGGTTTCTTTGTTTTTTTCTATGTTTATATCAGTCCTAATGCAAT
TTGATGACTGATGCAGAGTGTGCCGAGAGATTTCGTGAAGCGAAGTGGTTTTAGTAAAGG
AAGGCATGAGATAGTTTTGATGAACGAAGAAAGAAGTCATGGGATCAGAAGTAAAGAGTA
TATGTCT

Last Updated on 06.12.2018 16:11