Sequences from confirmation amplicon


Confirmation sequences for line 213D09 and hit At4g33430 are shown.
Line ID 213D09
Locus At4g33430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16089719
Primers used for PCR MG25 - 8474, show primer details
   
Sequence Name 78-K031454-022-213-D09C-MG25
GenBank Accession LN513626 [GenBank]
Sequencing primer gene specific primer, MG25: 5'-TGTTCTCACCTAAAGAAGGCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K031454-022-213-D09C-MG25
TGCTGTATTGACTTGAGTATATTTGGGTTACATCTGTTTCGACTGATTCCAGTGATTATG
TATCCTCGTGACACTTGATGATTGCAAAGTTTGATTTTTTCTTCTTCTTTGGTTAATTGT
AGGTGATGCTCTAAGTGCACTGAAAAACAGTTTAGCCGACCCTAATAAGGTGCTTCAAAG
TTGGGATGCTACTCTTGTTACTCCATGTACATGGTTTCATGTTACTTGCAATAGCGACAA
TAGTGTTACACGTGTGTAAGTTTCTTTCTATATCTAGCCAAGATGATTCGGATTTTCGCA
TCTACTGAGAATTTACTTGAACTTCATTTTTGCTCAGTCCGGGAAATATTCAATTGAAGC
CATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCACTCCAAAATGG
   
Sequence Name 70-K031454-022-213-D09C-8409
GenBank Accession LN512307 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K031454-022-213-D09C-8409
ATTTACAATTGAATATACATGGCTTCAATTGAATATTTCCCGGACTGAGCAAAAATGAAG
TTCAAGTAAATTCTCAGTAGATGCGAAAATCCGAATCATCTTGGCTAGATATAGAAAGAA
ACTTACACACGTGTAACACTATTGTCGCTATTGCAAGTAACATGAAACCATGTACATGGA
GTAACAAGAGTAGCATCCCAACTTTGAAGCACCTTATTAGGGTCGGCTAAACTGTTTTTC
AGTGCACTTAGAGCATCACCTACAATTAACCAAAGAAGAAGAAAAAATCAAACTTTGCAA
TCATCAAGTGTCACGAGGATACATAATCGCTGGAATCAGTCGAAACAGATGTAACCCAAA
TATAACTCAAGTCAATACAGCAAAACAAGTAACAGATAAGTTAAAAATGCCTTCTTTAGG
TGAGAAC

Last Updated on 06.12.2018 16:11