Sequences from confirmation amplicon


Confirmation sequences for line 903G11 and hit At4g34660 are shown.
Line ID 903G11
Locus At4g34660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16548508
Primers used for PCR 80ns - 8474, show primer details
   
Sequence Name 45-K114649-0022-903-G11C-80ns
GenBank Accession LN505620 [GenBank]
Sequencing primer gene specific primer, 80ns: 5'-TACAGAGACTGGAGAAACAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114649-0022-903-G11C-80ns
CGACAGCCAGGTTAAGGGTCTTTCTAGATGGTGTAGCGACGACGAGCTACAGTGAGGAAG
AAGAAGAAGCGGCTTCAACAAGTGAGAGAAAGCCCTAATTCACTGGCTTTTCATTTATAT
ATGTCTAGGGTTTTGCGTCTTTTTAGTTATTTTGAGATGATATAAATTTATAGCTTACTG
GATTTTATTTTCAAGCCCAATGGGCTATTATAGAGAGCCCAATATTTTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 16-K114648-0022-903-G11C-8409
GenBank Accession LN493530 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114648-0022-903-G11C-8409
CTCTCTATAATAGCCCATATGGGCTCGAAAATAAAATCCAGTAAGCTATAAATTTATATC
ATCTCAAAATAACTAAAAAGACGCAAAACCCTAGACATATATAAATGAAAAGCCAGTGAA
TTAGGGCTTTCTCTCACTTGTTGAAGCCGCTTCTTCTTCTTCCTCACTGTAGCTCGTCGT
CGCTACACCATCTAGAAGACCCTTAACCATGGCTGTCGGGTAAATCTCTCTTCGCATCTC
TATATTCCTTGCTTGTTTCCGNNTTCTCTGT
Primers used for PCR 81ns - 8474, show primer details
   
Sequence Name 49-K114649-0022-903-G11C-81ns
GenBank Accession LN506928 [GenBank]
Sequencing primer gene specific primer, 81ns: 5'-AGAACTTTAACCTGCTGCTGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K114649-0022-903-G11C-81ns
ATTCTTCACCAGATCAGAGCTATTCACAAATCTTCTTCCTTCTCTCTTTCTCCTATTCTT
CCACTTTACGAGTTCCCTCCGATTACTTGGAACAACTCAGAGAAGACAGAGATTCGAAAT
CGTCAAAAATGGCGTTGAGCTCAGAACAAATTTCTCTGCTTTCGAACACGAAGAAATCAA
AGATAGATTAAACCGAATGCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 20-K114648-0022-903-G11C-oriL
GenBank Accession LN495318 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114648-0022-903-G11C-oriL
CTTTACATCGAAATGCATTCGGTTTAATCCTATCTTTGATTTCTTCGTGTTCGAAAGCAG
AGAAATTTGTTCTGAGCTCAACGCCATTTTTGACGATTTCGAATCTCTGTCTTCTCTGAG
TTGTTCCAAGTAATCGGAGGGAACTCGTAAAGTGGAAGAATAGGAGAAAGAGAGAAGGAA
GAAGATTTGTGAATAGCTCTTGATCTGGTGAAGAATGGATGCAATTAGAAAACAAGCTAG
CAGGTTGAGAGAACAAGTTGCGAGACAGCAGCAGTTTAAAGTT
Primers used for PCR 14wl - 8474, show primer details
   
Sequence Name 79-K118446-0022-903-G11C-14wl
GenBank Accession LN513877 [GenBank]
Sequencing primer gene specific primer, 14wl: 5'-AGCTTGTTTTCTAATTGCATCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K118446-0022-903-G11C-14wl
ATCTTCTCCTTCTCTCTTTCTCCTATTCTTCCACTTTACGAGTTCCCTCCGATTACTTGG
AACAACTCAGAGAAGACAGAGATTCGAAATCGTCAAAAATGGCGTTGAGCTCAGAACAAA
TTTCTCTGCTTTCGAACACGAAGAAATCAAAGATAGATTAAACCGAATGCATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTAT
   
Sequence Name 39-K118445-0022-903-G11C-8409
GenBank Accession LN503518 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K118445-0022-903-G11C-8409
TTCATTGAATATGCATTCGGTTTAATCTATCTTTGATTTCTTCGTGTTCGAAAGCAGAGA
AATTTGTTCTGAGCTCAACGCCATTTTTGACGATTTCGAATCTCTGTCTTCTCTGAGTTG
TTCCAAGTAATCGGAGGGAACTCGTAAAGTGGAAGAATAGGAGAAAGAGAGAAGGAAGAA
GATTTGTGAATAGCTCTTGATCTGGTGAAGAATGGATGCAATTAGAAAACAAGCT
Primers used for PCR 28wm - 8474, show primer details
   
Sequence Name 19-K118502-0022-903-G11C-28wm
GenBank Accession LN494967 [GenBank]
Sequencing primer gene specific primer, 28wm: 5'-CAATCTCACATCGAATCAACGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118502-0022-903-G11C-28wm
ATCCGAAAAAAGCTCGCCCCTTTATCATCATCACATTATTGATTCATTCACCAGAAACCA
ACGACTCAACGGAATCACAAGAGAAGATAAAAACAAAATCTAGAAAAAAAGATACAGAAT
CGGGATCTAAGCAAATGGGCAAACCAAAAGTTAAGAACTTTTAACCTGCTGCTGTCTCGC
AACTTGTTCTCTCAACCTGCTAGCTTGTTTTCTAATTGCATCCATTCTTCACCAGATCAA
GAGCTATTCACAAATCTTCTTCCTTCTCTCTTTCTCCTATTCTTCCACTTTACGAGTTCC
CTCCGATTACTTGGAACAACTCAGAGAAGACAGAGATTCGAAATCGTCAAAAATGGCGTT
GAGCTCAGAACAAATTTCTCTGCTTTCGAACACGAAGAAATCAAAGATAGATTAAACCGA
ATGCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGAT
   
Sequence Name 03-K118501-0022-903-G11C-8409
GenBank Accession LN487687 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118501-0022-903-G11C-8409
AAAGCAGAGAAATTTGTTCCTGAGCTCAACGCCATTTTTGACGAATTTCGAATCTCTGTC
TTCTCTGAGTTGTTCCAAGTAATCGGAGGGAACTCGTAAAGTGGAAGAATAGGAAAAAAA
AGAGAAGGAAGAAGATTTGTGAATAGCTCTTGATCTGGTGAAGAATGGATGCAATTAGAA
AACAAGCTAGCAGGTTGAGAGAACAAGTTGCGAGACAGCAGCAGGTTAAAGTTCTTAACT
TTTGGTTTGCCCATTTGCTTAGATCCCGATTCTGTATCTTTTTTTCTAGATTTTGTTTTT
ATCTTCTCTTGTGATTCCGTTGAGTCGTTGGTTTCTGGTGAATGAATCAATAATGTGATG
ATGATAAAGGGGCTAGCTTTTTTTCGGATCGATGGTTTTTGACTTAATGAGTAATTGGAT
CTGAGTGTGATTAATACGTTGATT
Primers used for PCR 61xk - 8474, show primer details
   
Sequence Name 28-K119110-0022-903-G11C-61xk
GenBank Accession LN499067 [GenBank]
Sequencing primer gene specific primer, 61xk: 5'-CTTGGAACATGTAGCACCAATATG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K119110-0022-903-G11C-61xk
CTTTAATCCAGCTCACAAAACTGAAACGGATTCTCACATAAAAGTATCCTAAATTACCCT
TTGACAAAAACTTCTCCAATTCTACAGAATTTTACCCAATCTCACATCGAATCAACGTAT
TAATCACACTCAGATCCAATTACTCATTAAGTCAAAAACCATCGATCCGAAAAAAAGCTA
GCCCCTTTATCATCATCACATTATTGATTCATTCACCAGAAACCAACGACTCAACGGAAT
CACAAGAGAAGATAAAAACAAAATCTAGAAAAAAAGATACAGAATCGGGATCTAAGCAAA
TGGGCAAACCAAAAGTTAAGAACTTTAACCTGCTGCTGTCTCGCAACTTGTTCTCTCAAC
CTGCTAGCTTGTTTTCTAATTGCATCCATTCTTCACCAGATCAAGAGCTATTCACAAATC
TTCTTCCTTCTCTCTTTCTCCTATTCTTCCACTTTACGAGTTCCCTCCGATTACTTGGAA
CAACTCAGAGAAGACAGAGATTCGAAATCGTCAAAAATGGCGTTGAGCTCAGAACAAATT
TCTCTGCTTTCGAACACGAAGAAATCAAAGATAGATTAAACCGAATGCATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCATAAATGTAGATGTCCGC
   
Sequence Name 05-K119109-0022-903-G11C-8409
GenBank Accession LN488575 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119109-0022-903-G11C-8409
TCTATCTTTGATTTCTTCGTGTTCGAAAGCAGAGAAATTTGTTCTGAGCTCAACGCCATT
TTTGACGATTTCGAATCTCTGTCTTCTCTGAGTTGTTCCAAGTAATCGGAGGGAACTCGT
AAAGTGGAAGAATAGGAGAAAGAGAGAAGGAAGAAGATTTGTGAATAGCTCTTGATCTGG
TGAAGAATGGATGCAATTAGAAAACAAGCTAGCAGGTTGAGAGAACAAGTTGCGAGACAG
CAGCAGGTTAAAGTTCTTAACTTTTGGTTTGCCCATTTGCTTAGATCCCGATTCTGTATC
TTTTTTTCTAGATTTTGTTTTTATCTTCTCTTGTGATTCCGTTGAGTCGTTGGTTTCTGG
TGAATGAATCAATAATGTGATGATGATAAAGGGGCTAGCTTTTTTTCGGATCGATGGTTT
TTGACTTAATGAGTAATTGGATCTGAGTGTGATTAATACGTTGATTCGATGTGAGATTGG
GTAAAATTCTGTAGAATTGGAGAAGTTTTTGTCAAAGGGTAATTTAGGATACTTTTATGT
GAGAATCCGTTTCAGTTTTGTGAGCTGGGATTTGAAAGATGTTGTAGTGATAGATTAACA
TATTGGTGCTACATGTTCCA
Primers used for PCR 51yc - 8474, show primer details
   
Sequence Name 24-K119666-0022-903-G11C-51yc
GenBank Accession LN497215 [GenBank]
Sequencing primer gene specific primer, 51yc: 5'-GGTTGTGAAATCCATACCCTACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K119666-0022-903-G11C-51yc
ATAACGCATCTGTATCATATGCCAGTAAGGAGTGGTCTTTATCTTACCCAGAACTGGGTC
AACACATTCCTTCCCTGAACATCTTCAGCTCTAAGACGGATCTTCCTGTAGGCATTATCC
TCATCATTTTGTAGATCAGCAAGAGAAACCTCAAACACCCTGTGTTTCAGTCCCTCAGAG
GCAATCTGTTTAAATTAAGAGATAGCCCAGATAACAAAGTCAGATTTGTCGAAGAGGAAC
ACGCTCTTTTCTATCACAAATGCAATTCCTATTACTTCAATAATGAAAGCCACCAAGAGC
AAATGCCCATCTCTACACCTCACATTCAGCAAATGACCTATGCCATGAGATAACTATAGA
CTCAAAACCTAGAAAGCTCTAACCTTTCTCCAGTATAGTAATAGAAAGACATAAGACATT
ACCTTGGTACCCTGAGTCCTGGAAACAAGAGTCTTCCCAACATTCCTGTTCGTGAAAGAA
CCAGGAGCCTTCACGTCATACCAATCCTTCTTGGAGAAGGGATCAACACTGCACCATCCA
TAACAATTCAATCACGAACACTATATACAGATTACAATTAGAAATCTGAGAATTTTATTC
AGGACATAGGTTACTTACGCCTTCTTCTTTCCTCCTTTCCTACCCTTTGAAATCCTCTTG
TTCTTCCTATATGATCAAACAAAAACCTTAAATCAGAACATGATATACTCGAAGCTATGA
TCAAATGAGAGATTCCAATACAGAGACTGGAGAAACAAGCAAGGAATATAGAGATGCGAA
GAGAGATTTACCCGACAGCCATGGTTAAGGGTCTTCTAGATGGTGTAGCGACGACAAGCT
ACAGTGAGGAAGAGGAAGAAGCGGCTTCAACAAGTGAGAGAAAGCCCTATTCACTGGCTT
TTCATTTATATATGTCTAGGGTTTTGCGTNCTTTTAGTTATTTTGAGATGATATAAATTT
ATAGCTTACTGGATT
   
Sequence Name 08-K119665-0022-903-G11C-8409
GenBank Accession LN489955 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119665-0022-903-G11C-8409
ATTGAAATATTGGGCTCTCTATAATAGCCCATTGGGCTTGAAAATAAAATCCAGTAAGCT
ATAAATTTATATCATCTCAAAATAACTAAAAAGACGCAAAACCCTAGACATATATAAATG
AAAAGCCAGTGAATTAGGGCTTTCTCTCACTTGTTGAAGCCGCTTCTTCTTCTTCCTCAC
TGTAGCTCGTCGTCGCTACACCATCTAGAAGACCCTTAACCATGGCTGTCGGGTAAATCT
CTCTTCGCATCTCTATATTCCTTGCTTGTTTCTCCAGTCTCTGTATTGGAATCTCTCATT
TGATCATAGCTTCGAGTATATCATGTTCTGATTTAAGGTTTTTGTTTGATCATATAGGAA
GAACAAGAGGATTTCAAAGGGTAGGAAAGGAGGAAAGAAGAAGGCGTAAGTAACCTATGT
CCTGAATAAAATTCTCAGATTTCTAATTGTAATCTGTATATAGTGTTCGTGATTGAATTG
TTATGGATGGTGCAGTGTTGATCCCTTCTCCAAGAAGGATTGGTATGACGTGAAGGCTCC
TGGTTCTTTCACGAACAGGAATGTTGGGAAGACTCTTGTTTCCAGGACTCAGGGTACCAA
GGTAATGTCTTATGTCTTTCTATTACTATACTGGAGAAAGGTTAGAGCTTTCTAGGTTTT
GAGTCTATAGTTATCTCATGGCATAGGTCATTTGCTGAATGTGAGGTGTAGAGATGGGCA
TTTGCTCTTGGTGGCTTTCATTATTGAAGTAATAGGAATTGCATTTGTGATAGAAAAGAG
CGTGTTCCTCTTCGACAAATCTGACTTTGTTATCTGGGCTATCTCTTAATTTAACAGATT
GCCTCTGAGGGACTGAAACAAGGGTGTTTGAGGTTTCTCTTGCTGATCTACAAAATGATG
AGGATAATGCCT

Last Updated on 06.12.2018 16:11