Sequences from confirmation amplicon


Confirmation sequences for line 903G11 and hit At4g34670 are shown.
Line ID 903G11
Locus At4g34670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16548550
Primers used for PCR 51yc - 8474, show primer details
   
Sequence Name 24-K119666-0022-903-G11C-51yc
GenBank Accession LN497215 [GenBank]
Sequencing primer gene specific primer, 51yc: 5'-GGTTGTGAAATCCATACCCTACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K119666-0022-903-G11C-51yc
ATAACGCATCTGTATCATATGCCAGTAAGGAGTGGTCTTTATCTTACCCAGAACTGGGTC
AACACATTCCTTCCCTGAACATCTTCAGCTCTAAGACGGATCTTCCTGTAGGCATTATCC
TCATCATTTTGTAGATCAGCAAGAGAAACCTCAAACACCCTGTGTTTCAGTCCCTCAGAG
GCAATCTGTTTAAATTAAGAGATAGCCCAGATAACAAAGTCAGATTTGTCGAAGAGGAAC
ACGCTCTTTTCTATCACAAATGCAATTCCTATTACTTCAATAATGAAAGCCACCAAGAGC
AAATGCCCATCTCTACACCTCACATTCAGCAAATGACCTATGCCATGAGATAACTATAGA
CTCAAAACCTAGAAAGCTCTAACCTTTCTCCAGTATAGTAATAGAAAGACATAAGACATT
ACCTTGGTACCCTGAGTCCTGGAAACAAGAGTCTTCCCAACATTCCTGTTCGTGAAAGAA
CCAGGAGCCTTCACGTCATACCAATCCTTCTTGGAGAAGGGATCAACACTGCACCATCCA
TAACAATTCAATCACGAACACTATATACAGATTACAATTAGAAATCTGAGAATTTTATTC
AGGACATAGGTTACTTACGCCTTCTTCTTTCCTCCTTTCCTACCCTTTGAAATCCTCTTG
TTCTTCCTATATGATCAAACAAAAACCTTAAATCAGAACATGATATACTCGAAGCTATGA
TCAAATGAGAGATTCCAATACAGAGACTGGAGAAACAAGCAAGGAATATAGAGATGCGAA
GAGAGATTTACCCGACAGCCATGGTTAAGGGTCTTCTAGATGGTGTAGCGACGACAAGCT
ACAGTGAGGAAGAGGAAGAAGCGGCTTCAACAAGTGAGAGAAAGCCCTATTCACTGGCTT
TTCATTTATATATGTCTAGGGTTTTGCGTNCTTTTAGTTATTTTGAGATGATATAAATTT
ATAGCTTACTGGATT
   
Sequence Name 08-K119665-0022-903-G11C-8409
GenBank Accession LN489955 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119665-0022-903-G11C-8409
ATTGAAATATTGGGCTCTCTATAATAGCCCATTGGGCTTGAAAATAAAATCCAGTAAGCT
ATAAATTTATATCATCTCAAAATAACTAAAAAGACGCAAAACCCTAGACATATATAAATG
AAAAGCCAGTGAATTAGGGCTTTCTCTCACTTGTTGAAGCCGCTTCTTCTTCTTCCTCAC
TGTAGCTCGTCGTCGCTACACCATCTAGAAGACCCTTAACCATGGCTGTCGGGTAAATCT
CTCTTCGCATCTCTATATTCCTTGCTTGTTTCTCCAGTCTCTGTATTGGAATCTCTCATT
TGATCATAGCTTCGAGTATATCATGTTCTGATTTAAGGTTTTTGTTTGATCATATAGGAA
GAACAAGAGGATTTCAAAGGGTAGGAAAGGAGGAAAGAAGAAGGCGTAAGTAACCTATGT
CCTGAATAAAATTCTCAGATTTCTAATTGTAATCTGTATATAGTGTTCGTGATTGAATTG
TTATGGATGGTGCAGTGTTGATCCCTTCTCCAAGAAGGATTGGTATGACGTGAAGGCTCC
TGGTTCTTTCACGAACAGGAATGTTGGGAAGACTCTTGTTTCCAGGACTCAGGGTACCAA
GGTAATGTCTTATGTCTTTCTATTACTATACTGGAGAAAGGTTAGAGCTTTCTAGGTTTT
GAGTCTATAGTTATCTCATGGCATAGGTCATTTGCTGAATGTGAGGTGTAGAGATGGGCA
TTTGCTCTTGGTGGCTTTCATTATTGAAGTAATAGGAATTGCATTTGTGATAGAAAAGAG
CGTGTTCCTCTTCGACAAATCTGACTTTGTTATCTGGGCTATCTCTTAATTTAACAGATT
GCCTCTGAGGGACTGAAACAAGGGTGTTTGAGGTTTCTCTTGCTGATCTACAAAATGATG
AGGATAATGCCT

Last Updated on 06.12.2018 16:11