Sequences from confirmation amplicon


Confirmation sequences for line 778F10 and hit At4g35030 are shown.
Line ID 778F10
Locus At4g35030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16677273
Primers used for PCR NF29 - 8474, show primer details
   
Sequence Name 05-K102429w-0022-778-F10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K102429w-0022-778-F10C-8409
ATTTACAATTGAATATTTCCCACCACATCACCTTGTATCGAATATCGAGAAGATGTTGTC
GGTCCCCACATCGACAGACCAAAATCTGACAGCTATTCTCATAATCATAAGAAATACACT
CGGTCTCTTATGAAAATTAACATATAAATCAAAGCTAGAAGCTTACATTACCTGAGGTTG
GAGCTCAAGTGATAGAAGGACGTTAGAAGTTTTGACATCTCTATGAATCACTGGCTTAGA
ACATCGGTTATGAAGATAGTCTAATGCTTCCGCCAACCCGATAGCTATCTTGAATCTCTC
TTCCCATGATAATACATACTTTCCCTTTTGCTTACCTG
   
Sequence Name 21-K102430-0022-778-F10C-NF29
GenBank Accession LN495609 [GenBank]
Sequencing primer gene specific primer, NF29: 5'-CAGGTAAGCAAAAGGGAAAGTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K102430-0022-778-F10C-NF29
AGATAGCTATCGGGTTGGCGGAGCATTAGACTATCTTCATAACCGATGTTCTAAGCCAGT
GATTCATAGAGATGTCAAAACTTCTAACGTCCTTCTATCACTTGAGCTCCAACCTCAGGT
AATGTAAGCTTCTAGCTTTGATTTATATGTTAATTTTCATAAGAGACCGAGTGTATTTCT
TATGATTATGAGAATAGCTGTCAGATTTTGGTCTGTCGATGTGGGGACCGACAACATCTT
CTCGATATTCGATACAAGGTGATGTGGTGGGAAATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11