Sequences from confirmation amplicon


Confirmation sequences for line 180B08 and hit At4g36170 are shown.
Line ID 180B08
Locus At4g36170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17116814
Primers used for PCR 73za - 8474, show primer details
   
Sequence Name 20-K123982-0022-180-B08C-73za
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 73za: 5'-TATTAATGCTAAACGCATGCTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K123982-0022-180-B08C-73za
TAGGAAGAGCATATGTCTCTTTTCAATAATAAGTTAATCTCATTTGTAACCTTTATCTAT
AAATATTGGGTTGATTTTTTGTGTCTTCACATTTTTTTTTAGATATCAAATGTTGTTTTT
TTCTAAAATATATATTAAACTTCGTACGGTATGAGACACATAATTCATTTTGCATATTGA
TAAATAGAAATAATGATTGTAGGGATCTAAATACGAAAAAACACATTATTATAATAATCT
CGATAGATGAATATATAAAGTAATTATTTATAGTTCTTGCTTGAGTCATGTTCTATTGGT
ATTGGCATGCCGACGGAATCCATTAAAACAAAAGTCGAATTTGTTATGGTGAAATTGTTA
TTAATTCTTCTATAAATGGTGAGATTTTAAGGTGAAACTTGCTGTTTGTCTTCTTCACTT
GTTGTGTTGGCCGTTAAGGTGAAAATAATAATGACTTGGTACTTTGTAAGTGAAAAATGA
GGGAATAATATGGATCAGCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAG
   
Sequence Name 07-K123981-0022-180-B08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K123981-0022-180-B08C-8409
TGATCATATTATTCCCTCATTTTTCACTTACAAAGTACCAAGTCATTATTATTTTCACCT
TAACGGCCAACACAACAAGTGAAGAAGACAAACAGCAAGTTTCACCTTAAAATCTCACCA
TTTATAGAAGAATTAATAACAATTTCACCATAACAAATTCGACTTTTGTTTTAATGGATT
CCGTCGGCATGCCAATACCAATAGAACATGACTCAAGCAAGAACTATAAATAATTACTTT
ATATATTCATCTATCGAGATTATTATAATAATGTGTTTTTTCGTATTTAGATCCCTACAA
TCATTATTTCTATTTATCAATATGCAAAATGAATTATGTGTCTCATACCGTACGAAGTTT
AATATATATTTTAGAAAAAAACAACATTTGATATCTAAAAAAAAATGTGAAGACACAAAA
AATCAACCCAATATTTATAGATAAAGGTTACAAATGAGATTAACTTATTATTGAAAAGAG
ACATATGCTCTTTCCTAATGTAACAGCCTAACAGAATAACAAGATAATCCCTAGCATGCG
TTAGCCATTAA

Last Updated on 06.12.2018 16:11