Sequences from confirmation amplicon


Confirmation sequences for line 281D11 and hit At4g40050 are shown.
Line ID 281D11
Locus At4g40050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18563354
Primers used for PCR 13vf - 8474, show primer details
   
Sequence Name 21-K118126-0022-281-D11C-13vf
GenBank Accession LN495852 [GenBank]
Sequencing primer gene specific primer, 13vf: 5'-TTCCAGAAAAACAAACAAACAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118126-0022-281-D11C-13vf
CTTGCCAGAGAGCATCACGAAACTTAAACAATTTCTTAGCGTGGAAGCGCGCAAGATAAG
TTTGAGAAGCTGGATATGAATCAAGCATTGCACAGTAACGTAGAGGCCTGAGATAAGTTA
AATTTGTATCAGATTCTATAAAGCGAGTGATTTCTTGCACAACTAGCCTCCACTCCTTAA
AGTTTGTCTCCTACACAAGAGAAGAAATATGTATAAAAAAAGAGACTTGAACCAATATAA
ATCACAACAAGGGTCAGATCAAAAGTACCCTAAAATTGGATATGCTGTGATCAACCAAAA
GCCTAAGCTTGTCAGCTAAGTGCAAAAGCATTTGTTTCCGATCAACAATCAACGAAACAA
GCAGAAACCTGGCATAGAAGCTAAGTAAATGGTTTCTGATAATTAGTATAAAATGAAATA
CGTACGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTAT
   
Sequence Name 05-K118125-0022-281-D11C-8409
GenBank Accession LN488545 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118125-0022-281-D11C-8409
ATTATAGAAACCATTTACTTAGCTTCTATGCCAGGTTTCTGCTTGTTTCGTTGATTGTTG
ATCGGAAACAAATGCTTTTGCACTTAGCTGACAAGCTTAGGCTTTTGGTTGATCACAGCA
TATCCAATTTTAGGGTACTTTTGATCTGACCCTTGTTGTGATTTATATTGGTTCAAGTCT
CTTTTTTTATACATATTTCTTCTCTTGTGTAGGAGACAAACTTTAAGGAGTGGAGGCTAG
TTGTGCAAGAAATCACTCGCTTTATAGAATCTGATACAAATTTAACTTATCTCAGGCCTC
TACGTTACTGTGCAATGCTTGATTCATATCCAGCTTCTCAAACTTATCTTGCGCGCTTCC
ACGCTAAGAAATTGTTTAAGTTTCGTGATGCTCTCTTGGCAAGTTATCACCGTAATGAGG
TAAACAAGCACTGTTTGTTTGTTTTTCTGGA

Last Updated on 06.12.2018 16:11