Sequences from confirmation amplicon


Confirmation sequences for line 584G10 and hit At5g01300 are shown.
Line ID 584G10
Locus At5g01300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:121942
Primers used for PCR 18dj - 8474, show primer details
   
Sequence Name 20-K109341-0022-584-G10C-18dj
GenBank Accession LN495248 [GenBank]
Sequencing primer gene specific primer, 18dj: 5'-AACGAACTAATAATTGTTTTGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K109341-0022-584-G10C-18dj
GCTAATATCTACTGGTTATACACATTACATCTAGTTTGTTTCCCAACGGAATTTGAAACT
AGAATTATTTCATTGATAATCCTCAATACGTGATATGGAATTATTAACTATGATACATAT
CCAACGGTGTTATGACTATAACGACGAACGACATATTTTAGGCCAAACACGTCAAGATGG
CTTCTCCTAGCACGTGCCCTTCAATTGCGATCAGCAATCTCTCTTTCGTCACCTAGCTCA
TATTCAGAACAACTATTTATAAAAAAAAGTACTACATAAAGTACTACATATCATTCAAAT
CAGATAGGAAAATAATAAAACGATGTAGTACCGTGTGACCAATCTTGGGTTTATCATCGA
GAGCAAAAAGCTTGAACTGAAAACGGTGACCGTGACTAGGTAAGAGAGGTCCACGCCATC
CCGGAATCTTGTGATCGTTGTTCCCTTCCCGAATACCTTTAATAATAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 04-K109340-0022-584-G10C-8409
GenBank Accession LN487958 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K109340-0022-584-G10C-8409
ATTTACAATTGAATTATCCTATTATTAAAGGTATTCGGGAAGGGAACAACGATCACAAGA
TTCCGGGATGGCGTGGACCTCTCTTACCTAGTCACGGTCACCGTTTTCAGTTCAAGCTTT
TTGCTCTCGATGATAAACCCAAGATTGGTCACACGGTACTACATCGTTTTATTATTTTCC
TATCTGATTTGAATGATATGTAGTACTTTATGTAGTACTTTTTTTTATAAATAGTTGTTC
TGAATATGAGCTAGGTGACGAAAGAGAGATTGCTGATCGCAATTGAAGGGCACGTGCTAG
GAGAAGCCATCTTGACGTGTTTGGCCTAAAATATGTCGTTCGTCGTTATAGTCATAACAC
CGTTGGATATGTATCATAGTTAATAATTCCATATCACGTATTGAGGATTATCAATGAAAT
AATTCTAGTTTCAAATTCCGTTGGGAAACAAACTAGATGTAATGTGTATAACCATGTAGA
TTATTTAGCTGTACAAGTGTGAGAGAAGAGAAACCGCCCAAAACAAT

Last Updated on 06.12.2018 16:11