Sequences from confirmation amplicon


Confirmation sequences for line 104H06 and hit At5g01370 are shown.
Line ID 104H06
Locus At5g01370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:152489
Primers used for PCR 09ui - 8474, show primer details
   
Sequence Name 45-K117093-0022-104-H06C-09ui
GenBank Accession LN505664 [GenBank]
Sequencing primer gene specific primer, 09ui: 5'-AAGCTTGTCAAGATACAACAGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K117093-0022-104-H06C-09ui
CTCGCCGAGGATGGGAATATCTCACGGAAAGAAACGGAAGCTTTGACTCTCCGGCACTGA
CGTTGACCGTCTTTCTCGTCTTCGTTTTCTTCTTCTTCCTCGTGACCTTTTAGGATGTAG
TCCATGAAGTTAACCGATTTGCTCCTTGTGACAGCCGAACCGGAATCATCAAAGTACCGG
AACCGTTTTGATCCGGGTGCGGGATGATCTAGCCCCATGAGTTTGGCTACAACGTTGGGA
GGAGGAGGAGGTGGTGGAGGGTGAAGAGAGACGTCATCGTTTTGGGTTTCGGTTACGGTT
TTGATCTTCATCCTCGACCACATGTTCTCCTGAAAAAAGT
   
Sequence Name 29-K117092-0022-104-H06C-8409
GenBank Accession LN499453 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117092-0022-104-H06C-8409
ATCCTGTTAATACCAAGAACTTGTTTGCACATTTTCACACACACTCCAACTTAAAGCACT
ATCATTATCACCATCATCATTATCAGCCATCACCATGACCCAACGAGCCAACCCTAAGAC
CGGCTGCCTCACGGCGGTAGTCCGCCGTCTTCTATGCTCTGGTAGCCAACAGACTCACCC
TTCCGACAACATCCTCGACTCTGACGAAACACTCCAACTACTAATCTCTTACGAAGAAAA
CGAAGAGCCAAAGAAAGAGATCAAAACCGAAACCGAAACCCAAAACGATGACGTCTCTCT
TCACCCTCCACCACCTCCTCCTCCTCCCAACGTTGTAGCCAAACTCATGGGGCTAGATCA
TCCCGCACCCGGATCAAAACGGTTCCGGTACTTTGATGATTCCGGTTCGGCTGTCACAAG
GAGCAAATCGGTTAACTTCATGGACTACATCCTAAAAGGTCACGAGGAAGAAGAAGAAAA
CGAAGACGAGAAAGACGGTCAACGTCAGTGCCGGAGAGTCAAAGCTTCCGTTTCTTTCCG
TGAGATAGTCCCAACCTCGGCGAGATGGAGCTCTAATAACCAACAAAAGAAACATGATTT
CCTGCTGTTGTATCTTGACAA

Last Updated on 06.12.2018 16:11