Sequences from confirmation amplicon


Confirmation sequences for line 586A06 and hit At5g01410 are shown.
Line ID 586A06
Locus At5g01410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:172739
Primers used for PCR 09yl - 8474, show primer details
   
Sequence Name 41-K122428-0022-586-A06C-09yl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 09yl: 5'-TTATTTTAGCTTTTATTCACACCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K122428-0022-586-A06C-09yl
GCCGATCCATAGATAAACGAGGACCGGCCAGAAATCGCTTCACCATTCCCAAATCTCTCT
TCCATTTTCTCCACACAAATTTCTCTTCAATCTCCGATAATGGAAGGAACCGGCGTTGTG
GCGGTGTACGGTAACGGTGCGATAACGGAGGCGAAGAAATCTCCCTTCTCCGTGAAGGTC
GGTTTGGCTCAGATGCTCCGTGGTGGTGTTATCATGGATGTCGTCAACGCCGAGCAAGCT
CGTATCGCCGAGGAGGCTGGTGCTTGCGCCGTCATGGCTTTGGAGCGTGTTCCTGCTGAT
ATCCGCGCTCAAGGAGGCGTCGCTCGTATGAGCGATCCACAAATGATTAAAGAAATCAAA
CAAGCCGTTACGATTCCGGTGATGGCTAAGGCTAGGATTGGTCATTTCGTTGAAGCTCAG
ATCCTTGAAGCAATTGGAATCGATTACATCGATGAGAGCGAGGTTTTGACTCTTGCTGAT
GAAGATCATCACATCAACAAGCATAATTTCCGGATCCCGTTCGTTTGCGGTTGCCGGAAT
CTCGGCGAGGCTCTGAGGAGGATCCGTGAAGGTGCGGCGATGATTAGGACCAAAGGTGAA
GCTGGAACCGGTAACATTATTGAAGCTGTGAGGCATGTGAGGTCTGTTAATGGTGACATT
AGGGTTTTGCGAAACATGGATGATGATGAGGTTTTCACTTTCGCTAAGAAATTAGCCGCT
CCGTACGATCTCGTGATGCAGACTAAGCAGCTTGGTCGTCTTCCTGTAGTCCAATTCGCC
GCCGGTGGAGTGGCTACTCCGGCTGATGCAGCTCTCATGATGCAGCTTGGATGTGATGGT
GTCTTTGTTGGTTCTGGTATCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAAATGGGGGAAAAGAAAGAGTAATTACCA
   
Sequence Name 17-K122427-0022-586-A06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K122427-0022-586-A06C-8409
ATTGAAGATACCAGAACCAACAAAGACACCATCACATCCAAGCTGCATCATGAGAGCTGC
ATCAGCCGGAGTAGCCACTCCACCGGCGGCGAATTGGACTACAGGAAGACGACCAAGCTG
CTTAGTCTGCATCACGAGATCGTACGGAGCGGCTAATTTCTTAGCGAAAGTGAAAACCTC
ATCATCATCCATGTTTCGCAAAACCCTAATGTCACCATTAACAGACCTCACATGCCTCAC
AGCTTCAATAATGTTACCGGTTCCAGCTTCACCTTTGGTCCTAATCATCGCCGCACCTTC
ACGGATCCTCCTCAGAGCCTCGCCGAGATTCCGGCAACCGCAAACGAACGGGATCCGGAA
ATTATGCTTGTTGATGTGATGATCTTCATCAGCAAGAGTCAAAACCTCGCTCTCATCGAT
GTAATCGATTCCAATTGCTTCAAGGATCTGAGCTTCAACGAAATGACCAATCCTAGCCTT
AGCCATCACCGGAATCGTAACGGCTTGTTTGATTTCTTTAATCATTTGTGGATCGCTCAT
ACGAGCGACGCCTCCTTGAGCGCGGATATCAGCAGGAACACGCTCCAAAGCCATGACGGC
GCAAGCACCAGCCTCCTCGGCGATACGAGCTTGCTCGGCGTTGACGACATCCATGATAAC
ACCACCACGGAGCATCTGAGCCAAACCGACCTTCACGGAGAAGGGAGATTTCTTCGCCTC
CGTTATCGCACCGTTACCGTACACCGCCACAACGCCGGTTCCTTCCATTATCGGAGATTG
AAGAGAAATTTGTGTGGAGAAAATGGAAGAGAGATTTGGGAATGGTGAAGCGATTTCTGG
CCGGTCCTCGTTTATCTATGGATCGGCTTTATAGTGAGAGTTTGT

Last Updated on 06.12.2018 16:11