Sequences from confirmation amplicon


Confirmation sequences for line 724A11 and hit At5g03070 are shown.
Line ID 724A11
Locus At5g03070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:721205
Primers used for PCR 21tg - 8474, show primer details
   
Sequence Name 35-K116770-0022-724-A11C-21tg
GenBank Accession LN501972 [GenBank]
Sequencing primer gene specific primer, 21tg: 5'-TCCATTCGGAAATTGGTAATAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116770-0022-724-A11C-21tg
GGACTAATATGAAATTATATGCAAATATGAGTCGGCAAAATTTTCGTGTTTCTTTTTTTT
GCTGGGCGGGCGCGAGGGAACGGATCCAAATTAACCAATTCGTTTTCGGCGAAATCCTAT
TCTTACTCCGGTGAAATTACCGAATCAAAAACCCTACTCTCGAAGAAACTTGCTGTGCTC
AAGCTTCTTCAATGGCGGATGATGGCTCCGCCTCTAATCGAAGGGACCCAATAAAGTCTT
CAGGTGATTTTCCTTCTGACTTTTACTGTAAACCCTCAACTTGATTTTGGCACCTGAGTA
GTGTTAGTGTAGTGTTTTTGTTTGCTTCTCGAGATAATTAGGTTAGTTGGTGATTAGGGT
AGGAACAAATTTAGATGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTC
   
Sequence Name 11-K116769-0022-724-A11C-8409
GenBank Accession LN491289 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116769-0022-724-A11C-8409
TTACATTGAAGCATCTAAATTTGTTCCTACCCTAATCACCAACTAACCTAATTATCTCGA
GAAGCAAACAAAAACACTACACTAACACTACTCAGGTGCCAAAATCAAGTTGAGGGTTTA
CAGTAAAAGTCAGAAGGAAAATCACCTGAAGACTTTATTGGGTCCCTTCGATTAGAGGCG
GAGCCATCATCCGCCATTGAAGAAGCTTGAGCACAGCAAGTTTCTTCGAGAGTAGGGTTT
TTGATTCGGTAATTTCACCGGAGTAAGAATAGGATTTCGCCGAAAACGAATTGGTTAATT
TGGATCCGTTCCCTCGCGCCCGCCCAGCAAAAAAAAGAAACACGAAAATTTTGCCGACTC
ATATTTGCATATAATTTCATATTAGTCCCTAGTATTTCCAAGTATACAATAAATAGCCCA
TTTAACTTTATTACCAATTTCCCGAATGG

Last Updated on 06.12.2018 16:11