Sequences from confirmation amplicon


Confirmation sequences for line 316B12 and hit At5g03775 are shown.
Line ID 316B12
Locus At5g03775 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:999001
Primers used for PCR 34rb - 8474, show primer details
   
Sequence Name 19-K115950-0022-316-B12C-34rb
GenBank Accession LN494906 [GenBank]
Sequencing primer gene specific primer, 34rb: 5'-CTACATGGCTCAAGTTTGTGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115950-0022-316-B12C-34rb
AAAAATTAAAAAAACATGATCAATTTTTATGTATGTTGTATCCTTCTTTCCTGAGAGAAG
ACTCTTTTAAAGCTATCCTTCCTGTTTTTGAAAAAAAAAAAAAAAAACCGGGCCCTTTTT
TTTTCCAAAAAACCTTTAATTCCCCCTTTCCTTCCAAAAAAAAAAAGGTCCCCCTGGCCT
TTAAAACCCCCCTTTTTTTTTTTAAAATAAAAAAACCCCATTTTTTTAAAAAAAGGGGGG
GGGGAAATTTTTTTAAAAAAAAATTTTAAAAACCAAAAAAAAAATTTGGGGTTTTTTTTT
CCCCCCCCAAAAAATTCCAAAGTTTTAAAAAATTTGCCCCATTTTTTCCCCCATTTTTTA
AAAACCCAAAAAAATTTTTTCCCAAAACTTTTTTTTTTTTTTGCCGGCCCCCCCCAAAAA
ACCCCCCGGGCCCAAAGGAAAAGGGGTTTTACCTTCAAACCAAAAAATGGGGGGCCCAAA
AAAAGGGGGACCCCCCCTTTTTTTTAAACCCAAGGGGGGGAACCCCCCGGGGACCCCCCG
GGGTTTTGGGGGCCAATTGGGAAAAAAAAAAAAAATTTTCCCCCTTTTTTTTTCCTTCAA
AAAAGGGAATTCCCCCCCCTTTTAAAAAAAAA
   
Sequence Name 09-K115949-0022-316-B12C-8409
GenBank Accession LN490337 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115949-0022-316-B12C-8409
TACTATCAGTGCTGATCCCATTTACTCGAACCCACAATCGTTTGATTAGAAGTCAAACGC
CTTATCCATTAGGCCACGGGTGCTTTGTTGGGTAAGCTAGCTAAATAACATATATAGTTC
TGTAAAATATTTTTGTTCGCTTTTAACAATTTGATGAATATACTTGCTCAACTCTTCTAA
CATTTTGAATTTTTATGTGTAGCAAATATAAAGCATTAATTTTTCTTTTCGTTTTATCAA
TTTTTTTTTCAAAAAATCTAACCACTACATTTATATTAAAAACTATGATATTATTAATTT
AATAATATAAAAGTTTGATTTCAATTGCAATGGATACATTTTTTTTTTTTAAAGAAAAAA
AAAAATTAATTGTTTTTTTAAAAAAAAACCAATAAGGTTTTTTTTTTTTTTTTTAAAAAA
GGGGGGGGGATTTTTTAAAAAATTTTTCCCCCGGAAAAAAGGGGACCCCCCCCCAAAAAA
TTGGGCGGGTTTTTTTAATTTTTTTTTGGGCCCCCAAAAAAAAAAAAAATTTGGGAAGGG
GGTAAAAACCTTTTTTTCAAAAATT

Last Updated on 06.12.2018 16:11