Sequences from confirmation amplicon


Confirmation sequences for line 170C01 and hit At5g04470 are shown.
Line ID 170C01
Locus At5g04470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1267383
Primers used for PCR MZ54 - 8474, show primer details
   
Sequence Name 09-K025042-022-170-C01C-8409
GenBank Accession LN490016 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K025042-022-170-C01C-8409
TTTCCCGGACATGAAGCCATTTCTCTAAGAAGATTTGTTCAGGCACACGCTAAAACACAC
AAACAAAAAAACAACTCTTCAAGAACATATACATTTAATATTTATATAAACAAAAAAGAA
TGGATCTTGATTTAATACAAGATCTGCCCATCTTGAATTTCCCACCAGCCATCAAGATCC
GAGCCAACACCAACAGAGATGATGACGGCGGCGGCTGCACCACTCCCACTTCTTCCGACC
ACAAGATTCCTCCCACCACAGCCACCACTCCTCCTCCTCCACCGCAGAAACCCCGTCCAC
CTTCCACACCGTCGTCTCTCGGCATCAGATCTTGCAAGAGAAAGCTTATGACGTCATTGT
CCAAGTATGAGATCATCGTCAACAAAGATGAGATCGAGCGGTTCTTCTCCTCTGTTTACA
ACCAAACGATGGCGTCATCCACCACGACGGCTATCACGGTGGCCAAGCGGCGAAGAAGTT
TCCGTTCTTGTTCACGAAGATGATCAATAATTGGACGTTTCATTAAAGGTTCCATAATTT
CGAATAGCTTTTTCGATTTTTATTTATTTTTATGTAATGTTTTTATCAACTTTACACGTC
GACCCACTCTATAAATATACATGTGCGATCGATCGATGGAATCTTTTTTTCTTTACTAAA
ATTTGCTAGCTGTTTTGATAACGAATGACCAAATTCCAGTAGTTTTATTGACATCGTAAA
TATATNTCCAGCGAGAGGCACATGCACAAGTATGTATATCATCTTTTGGA
   
Sequence Name 10-K025042-022-170-C01C-MZ54
GenBank Accession LN490489 [GenBank]
Sequencing primer gene specific primer, MZ54: 5'-TCATCATAACCCATGACACAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K025042-022-170-C01C-MZ54
ATAATTCAAACTATTGATCAATTCAAGAGATTCATATATCCTTATTAAGAAAGTACATCG
ATCAGCTAATGATGAAAATGTACTTTTTGGCCACTAAATAAAGTAAATGCTATAGGATTT
CATTGTTCAGTTTCACAACTTTCATCAGTCGATATATATAGGTTTTTTCTTTTATCCAAA
AGATGATATACATACTTGTGCATGTGCCTCTCGCTGGAAATATATTTACGATGTCAATAA
AACTACTGGAATTTGGTCATTCGTTATCAAAACAGCTAGCAAATTTTAGTAAAGAAAAAA
AGATTCCATCGATCGATCGCACATGTATATTTATAGAGTGGGTCGACGTGTAAAGTTGAT
AAAAACATTACATAAAAATAAATAAAAATCGAAAAAGCTATTCGAAATTATGGAACCTTT
AATGAAACGTCCAATTATTGATCATCTTCGTGAACAAGAACGGAAACTTCTTCGCCGCTT
GGCCACCGTGATAGCCGTCGTGGTGGATGACGCCATCGTTTGGTTGTAAACAGAGGAGAA
GAACCGCTCGATCTCATCTTTGTTGACGATGATCTCATACTTGGACAATGACGTCATAAG
CTTTCTCTTGCAAGATCTGATGCCGAGAGACGACGGTGTGGAAGGTGGACGGGGTTTCTG
CGGTGGAGGAGGAGGAGTGGTGGCTGTGGTGGAGGAATCTTGTGGTCGGAAGAAGTGGGA
GTGGTGCAGCCGCCGCCGTCATCATCTCT

Last Updated on 06.12.2018 16:11