Sequences from confirmation amplicon


Confirmation sequences for line 322C11 and hit At5g05280 are shown.
Line ID 322C11
Locus At5g05280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1565550
Primers used for PCR GC78 - 8474, show primer details
   
Sequence Name 47-K033741-022-322-C11C-8409
GenBank Accession LN506118 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K033741-022-322-C11C-8409
CCATTTACAATTGAAGCCAGCAAATGGTTCCCGGCGGGGTGATGACCTGGCAACGTAACA
GAGCTTCATGAGGTCACTTTCAATGTTGCAGTGTTTGCTTCACCTAAGTAGGTTTTCTTC
TTTCTGGTTTGGTATATATATTTTTGTTTTGTTTGTAAATTACTGTTTACAATGACTGAA
GTTTTATCCACGATTACTTATATAATCTTTCTACTCTTCTACGTTATTTCCTTTTTTTTT
TTTGCTTGGGAAGTCCAAAGCAAAATCAAACCTTAGGTTCGGAAAAACCAACGGCTAGTC
AAATGCTTCGGTTAACAACAACTATTTACTTTTTGAACTAAAATCCCATCAATTGTTCCT
CTGTATTTTGCTGCCCCGTATCTGCTTAGTACAACAAAAGCAAAGTCACACTACATCACA
AACCAATGACCAATCCCAAATCTTCTGTTTCACAAAAAAAA
   
Sequence Name 57-K033741-022-322-C11C-GC78
GenBank Accession LN509336 [GenBank]
Sequencing primer gene specific primer, GC78: 5'-TCGTTTTGTGAAACAGAAGATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K033741-022-322-C11C-GC78
GTGACTTTGCTTTTGTTGTACTAAGCAGATACGGAGCAGCAAACTACAGAGGAACAATCG
ATGCGATTTTAGTTCAAAAAGTAACTAGTCGTTGTTAACCGAAGCATCTGACTAGCCGTT
GGTTTATCCGAACCTAAGGTTCGATTTTGCTCTGGACTTCCCAAGCAAAAAAAAAAAAGG
AAATAACGTAAAAAAGTAAAAAGATTATATAAGTAATCGGGGATAAAACTTCAGTCATTG
TAAACAGTAATTTACAAACAAAACAAAAATATATATACCAAACCAAAAAAAAAAAAACCT
ACTTAGGTGAAGCAAACACTCAACATTGAAAGTGACCTCATGAAGCTCTGTTACTTTGCC
ACGTCATCACCCCGCCGGGAACCATTTGCGGGCTTCAATTGAAAATGGCTTCATGTCCGG
GAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAAAAAAAAAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAAATTTCCCCACCGTTTTTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACAATCCGTCGTATTTATGGGCAAAAGCAATAAACAA
ATTATTCTAATTCGAAAATCTTTATTTCAACGTGTCTACA

Last Updated on 06.12.2018 16:11