Sequences from confirmation amplicon


Confirmation sequences for line 504E06 and hit At5g05730 are shown.
Line ID 504E06
Locus At5g05730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1721906
Primers used for PCR 61ra - 8474, show primer details
   
Sequence Name 37-K115914-0022-504-E06C-61ra
GenBank Accession LN502739 [GenBank]
Sequencing primer gene specific primer, 61ra: 5'-CCACTTCATGAACAAGATAAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K115914-0022-504-E06C-61ra
GGGCATCATCCTTCATCTAAAAGCGCGAGAAAAGATATTCAAGAACGTAAAGGCGAATTG
GGCACAAAAGGTAGAGGGAAAACTAGATCACAACAGGGACAGAATGACCAGCATAAGAAA
GTTCTCTACTTTATCAATTGCTCTTGCCTCTTGGGAAATCAGACGTACATGTTTACAACT
AGGAATCCCATGACCCCATATGATATGGTATGTAGTGAACACGGGTTACGTGCATTAATT
GCTAAATGGTTTTGAGATAGAAATCCATGAAACTTGACATTTAAAAAGATCACTCAAAAG
CAAGAGCTTGTCTCGAAGACATCCATACCATTATCACATATCCCCAGTCCTTAAAACTAG
TGGCTTCATTCCTTTTAAGTTAACTATTCTAGAAAGTGCATTCACGTCATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 11-K115913-0022-504-E06C-8409
GenBank Accession LN491267 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115913-0022-504-E06C-8409
ATTTACATTGATATGACGTGAATGCACTTTCTAGAATAGTTAACTTAAAAGGAATGAAGC
CACTAGTTTTAAGGACTGGGGATATGTGATAATGGTATGGATGTCTTCGAGACAAGCTCT
TGCTTTTGAGTGATCTTTTTAAATGTCAAGTTTCATGGATTTCTATCTCAAAACCATTTA
GCAATTAATGCACGTAACCCGTGTTCACTACATACCATATCATATGGGGTCATGGGATTC
CTAGTTGTAAACATGTACGTCTGATTTCCCAAGAGGCAAGAGCAATTGATAAAGTAGAGA
ACTTTCTTATGCTGGTCATTCTGTCCCTGTTGTGATCTAGTTTTCCCTCTACCTTTTGTG
CCCAATTCGCCTTTACGTTCTTGAATATCTTTTCTCGCGCTTTTAGATAGAAGATTGATT
GCCCTTAGTGTTTTAGTTTACTTTTTGTGCTTTATCTT

Last Updated on 06.12.2018 16:11