Sequences from confirmation amplicon


Confirmation sequences for line 408C06 and hit At5g05920 are shown.
Line ID 408C06
Locus At5g05920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1778765
Primers used for PCR 26ij - 8474, show primer details
   
Sequence Name 34-K112667-0022-408-C06C-26ij
GenBank Accession LN501474 [GenBank]
Sequencing primer gene specific primer, 26ij: 5'-GCATCATATGGTTTGTGATTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K112667-0022-408-C06C-26ij
TTTTGAGTAAATTTCGAGCTTAGTTTTGATTTCATGGTTTTTCTGCAGGTTGATGTTATA
GTCACGACAACTGGTGGTGTTGAGGAAGATCTCATAAAATGCCTTGCACCTACATTTAAA
GGTGATTTCTCTCTACCTGGAGCTTATTTAAGGTCAAAGGGATTGAACCGAATTGGGAAT
TTGCTGGTTCCTAATGATAACTACTGCAAGTTTGAGGATTGGATCATTCCCATCTTTGAC
GAGATGTTGAAGGAACAGAAAGAAGAGGTATTGCTTTATCTTTCCTTTTTATATGATTTG
AGATGATTCTGTTTGTGCGTCACTAGTGGAGATAGATTTTGATTCCTCTCTTGCATCATT
GACTTCGTTGGTGAATCCTTCTTTCTCTGGTTTTTCCTTGTAGAATGTGTTGTGGACTCC
TTCTAAACTGTTACCAATTGGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 02-K112666-0022-408-C06C-8409
GenBank Accession LN487105 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112666-0022-408-C06C-8409
ACCAATTGGTAACGNTTTAGAAGGAGTCCACAACACATTCTACAAGGAAAAACCAGAGAA
AGAAGGATTCACCAACGAAGTCAATGATGCAAGAGAGGAATCAAAATCTATCTCCACTAG
TGACGCACAAACAGAATCATCTCAAATCATATAAAAAGGAAAGATAAAGCAATACCTCTT
CTTTCTGTTCCTTCAACATCTCGTCAAAGATGGGAATGATCCAATCCTCAAACTTGCAGT
AGTTATCATTAGGAACCAGCAAATTCCCAATTCGGTTCAATCCCTTTGACCTTAAATAAG
CTCCAGGTAGAGAGAAATCACCTTTAAATGTAGGTGCAAGGCATTTTATGAGATCTTCCT
CAACACCACCAGTTGTCGTGACTATAACATCAACCTGCAGAAAAACCATTGAAATCAAAA
CTAAAGCTCGAAAATTTTAACATCTATAAAAAAGCAGGGTGATAAAGCAAAAATCACAAA
CCATATGATG

Last Updated on 06.12.2018 16:11