Sequences from confirmation amplicon


Confirmation sequences for line 452B05 and hit At5g06180 are shown.
Line ID 452B05
Locus At5g06180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1875010
Primers used for PCR 52ns - 8474, show primer details
   
Sequence Name 26-K114626-0022-452-B05C-52ns
GenBank Accession LN498040 [GenBank]
Sequencing primer gene specific primer, 52ns: 5'-AAACAGTTGTGTGGTATGCTTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114626-0022-452-B05C-52ns
TATCTTTCTCAGAAGAACCCAAAGAAGGTTTGTAAAAGTCTCTTTAGAATGTCACGATCA
TGGGGAGCGGATGGTCATCATATACTCGATCTTTCTACAGGTTGTAGAAGTCATAACTGG
AGAATTGAGCTCTAACTCTAAGGTCTACATTTGGGATGGAAAAGGTAAACGGCTTTTTAC
TCTCAACTTGTCTGTGGCAAAGTTTGATGTTTTCTCTTCATCCATTTCTCAGACCCTAAT
CCGCATTTGGGACATCTAGCTTTAGCCAATACTTTTATCATAACTGCTGACTCTATAAGT
ATGTTGAGCGAGGCATGTAGTACCGGGTATGTTTAAGAAAACGACAATCTTTTCCTTTTC
ACACTCTTAAAATGATATCAAAATCTCATTCTCTACACAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 26-K114625-0022-452-B05C-8409
GenBank Accession LN498039 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114625-0022-452-B05C-8409
TCATTGAATATATCCTGTGTAGAGAATGAGATTTTGATATCATTTTAAGAGTGTGAAAAG
GAAAAGATTGTCGTTTTCTTAAACATACCCGGTACTACATGCCTCGCTCAACATACTTAT
AGAGTCAGCAGTTATGATAAAAGTATTGGCTAAAGCTAGATGTCCCAAATGCGGATTAGG
GTCTGAGAAATGGATGAAGAGAAAACATCAAACTTTGCCACAGACAAGTTGAGAGTAAAA
AGCCGTTTACCTTTTCCATCCCAAATGTAGACCTTAGAGTTAGAGCTCAATTCTCCAGTT
ATGACTTCTACAACCTGTAGAAAGATCGAGTATATGATGACCATCCGCTCCCCATGATCG
TGACATTCTAAAGAGACTTTTACAAACCTTCTTTGGTGTTCTTCTTGAGAAAGATATTCT
TAGGCTTCCACAGCTCCAGAGGATACTCTGAAGCATACCACAAAACTGTT

Last Updated on 06.12.2018 16:11