Sequences from confirmation amplicon


Confirmation sequences for line 265D03 and hit At5g07760 are shown.
Line ID 265D03
Locus At5g07760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2471814
Primers used for PCR 75pv - 8474, show primer details
   
Sequence Name 87-K115459-0022-265-D03C-75pv
GenBank Accession LN514793 [GenBank]
Sequencing primer gene specific primer, 75pv: 5'-CTTATGTAGTTACAAACAAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K115459-0022-265-D03C-75pv
TTTTTCTACTGGTTTGTAGATCGACCAGAGGAGAGCCTTTAACACTATGATTATGCTTCA
GAAAGTAGAGATGCCGCTGCCTGATATGATGGTAAGCTCCAAAACCTACTTTACTAGCGA
ACAAGTCATTACTGGTTAATGTTTTATAGTACAATGATAATTTATTCACATTATTCTGTG
CATCATTTAGGCTGCAGTTCTAGGAATGGATGAGTCTGTACTAGATGTTGATCAAATAGA
GAATCTTATAAGGTTTTGCCCAACAAAGGAGGAGATGAAGCTTCTTAAGGTTACCTCTTG
ACTTTTGAGTAGCACTATCTACTTCTGTCTTTACTTTTGTCAATTCTTGGTTATCATTAT
TCACTTGTTAGTTGACACGAGTCATTATTCTTGGTCGTAATTGATTGGTTTGTTGTAGAA
CTATACTGGTGACAAGGCAACCTTGGGAAAGTGTGAGCAGGTCTGTCTTTAAGTCTTTAG
ATTACCAATCTCACACTGTTTTTATTTTCCACTATAATATAGTTCTTAACAATTTGCTTA
CCATCTTGTCACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 38-K115458-0022-265-D03C-8409
GenBank Accession LN503097 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115458-0022-265-D03C-8409
CATTTCAATTGAATATATCCTGTGACAAGATGGTAAGCAAATTGTTAAGAACTATATTAT
AGTGGAAAATAAAAACAGTGTGAGATTGGTAATCTAAAGACTTAAAGACAGACCTGCTCA
CACTTTCCCAAGGTTGCCTTGTCACCAGTATAGTTCTACAACAAACCAATCAATTACGAC
CAAGAATAATGACTCGTGTCAACTAACAAGTGAATAATGATAACCAAGAATTGACAAAAG
TAAAGACAGAAGTAGATAGTGCTACTCAAAAGTCAAGAGGTAACCTTAAGAAGCTTCATC
TCCTCCTTTGTTGGGCAAAACCTTATAAGATTCTCTATTTGATCAACATCTAGTACAGAC
TCATCCATTCCTAGAACTGCAGCCTAAATGATGCACAGAATAATGTGAATAAATTATCAT
TGTACTATAAAACATTAACCAGTAATGACTTGTTCGCTAGTAAAGTAGGTTTTGGAGCTT
ACCATCATATCAGGCAGCGGCATCTCTACTTTCTGAAGCATAATCATAGTGTTAAAGGCT
CTCCTCTGGTCGATCTACAAACCAGTAGAAAAACCGTCCATCAAACAGAGTGATATATAT
ATTATTATTATTACGTCAATCACTTGTTT

Last Updated on 06.12.2018 16:11