Sequences from confirmation amplicon


Confirmation sequences for line 345E07 and hit At5g07790 are shown.
Line ID 345E07
Locus At5g07790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2485518
Primers used for PCR GD58 - 8474, show primer details
   
Sequence Name 66-K033712-022-345-E07C-oriL
GenBank Accession LN511535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K033712-022-345-E07C-oriL
ATTTACTGGGAATTGGATATCTCTTGTTGCAGAGGGAAATTATCACAAGTATCTGCAGCT
GAGGCTATAGTTGATATTTCTAGAAAGTCTGGTCGAGAAACTGCTGCTTGTATCACTTCC
TTAAGCAAAAATCTACTTTGGTTCGCCGACATTTCTTCCTCGGTTGCTGAGGACAACAAA
ATAGACACATTGCAGCTGACAGAAAAGAAGCTAGAGGAGCACGAGACAAATCTAAGGTTG
AACTCTTCAGACAACATTGCAGCAGTCTCCAGCATTGTCCTTAAAAAGCAGAGGAGAAGC
CGAGCCAGGCAAGGGAAGCGGAAATGCCAAGATGACCAGCACATTGTGGACACATTTTCA
GAATGGGAAGCTAATGATGATCTGCAGGTAATAGGAAAGCTAATGGAAGCGTCTGACTTA
AAATGGAATTGCTGGTTCCCTAAGAACAAGAGCAAAAGCTCTC
   
Sequence Name 82-K033712-022-345-E07C-GD58
GenBank Accession LN514137 [GenBank]
Sequencing primer gene specific primer, GD58: 5'-GGAGAGCTTTTGCTCTTGTTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K033712-022-345-E07C-GD58
TTAGTCGACGCTTCCATTAGCTTTCCTATTACCTGCAGATCATCATTAGCTTCCCATTCT
GAAAATGTGTCCACAATGTGCTGGTCATCTTGGCATTTCCGCTTCCCTTGCCTGGCTCGG
CTTCTCCTCTGCTTTTTAAGGACAATGCTGGAGACTGCTGCAATGTTGTCTGAAGAGTTC
AACCTTAGATTTGTCTCGTGCTCCTCTAGCTTCTTTTCTGTCAGCTGCAATGTGTCTATT
TTGTTGTCCTCAGCAACCGAGGAAGAAATGTCGGCGAACCAAAGTAGATTTTTGCTTAAG
GAAGTGATACAAGCAGCAGTTTCTCGACCAGACTTTCTAGAAATATCAACTATAGCCTCA
GCTGCAGATACTTGTTGATAATTTCCCTCTGCAACAAGAGATATCCAATTCCCATGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
GTCC

Last Updated on 06.12.2018 16:11