Sequences from confirmation amplicon


Confirmation sequences for line 516F08 and hit At5g08370 are shown.
Line ID 516F08
Locus At5g08370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2692113
Primers used for PCR 11ar - 8474, show primer details
   
Sequence Name 03-K106771-0022-516-F08C-8409
GenBank Accession LN487503 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K106771-0022-516-F08C-8409
ATGAATTGCATATTTCAGTTCTTGTTTCCTAAGATTGGGAAAAAACCATCAAAGAAGAGA
AACCTTTTCCAATTATCTTGGATATCTCCTGTTGTTCTCCAACTGTTGCCAATATCTCCT
GCCCAAGTTGCTGGATCCTCTTGTCCCCTGCACTTACAAGTCAATTTCTTGTTTAGTATC
ATCAGACCATAGTTGTCTGAATAAGTGAATCTTCTTCTTAATTAGTCTTTTTTACCATTC
GCACAGAGAGAAGAATATGGATCTTCCTGAATTTAACAGCGCTTTACTCATCTTAGGGTA
TCTTTCTCTTGGACTTGTCCCTGTATTTTCGCAGTTATCATACTTGAGGTAATCAATCCC
CTGTCGAATACATGATAACGAAAACGCACAGCTTCAAGGTTCAGGAAATGAGTTCAGACA
TAACACATACAAATATACATACCCATGAGGCA
   
Sequence Name 19-K106772-0022-516-F08C-11ar
GenBank Accession LN494777 [GenBank]
Sequencing primer gene specific primer, 11ar: 5'-TTGCCTCATGGGTATGTATATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K106772-0022-516-F08C-11ar
AGCTGTGCGTTTTCGTTATCATGTATTCGACAGGGGATTGATTACCTCAAGTATGATAAC
TGCGAAAATACAGGGACAAGTCCAAGAGAAAGATACCCTAAGATGAGTAAAGCGCTGTTA
AATTCAGGAAGATCCATATTCTTCTCTCTGTGCGAATGGTAAAAAAGACTAATTAAGAAG
AAGATTCACTTATTCAGACAACTATGGTCTGATGATACTAAACAAGAAATTGACTTGTAA
GTGCAGGGGACAAGAGGATCCAGCAACTTGGGCAGGAGATATTGGCAACAGTTGGAGAAC
AACAGGAGATATCCAAGATAATTGGAAAAGGTTTCTCTTCTTTGATGGTTTTTTCCCAAT
CTTAGTGAAACAAGAACTTGAAATATGCAATTATCAATCCACATATATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCACGGTTAT

Last Updated on 06.12.2018 16:11