Sequences from confirmation amplicon


Confirmation sequences for line 088G05 and hit At5g10340 are shown.
Line ID 088G05
Locus At5g10340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3253227
Primers used for PCR 10cs - 8474, show primer details
   
Sequence Name 65-K113193-0022-088-G05C-10cs
GenBank Accession LN511437 [GenBank]
Sequencing primer gene specific primer, 10cs: 5'-ATAGGAACCAACCATTGTCTTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K113193-0022-088-G05C-10cs
TTTTTGATTTGAGTCCACCCGAATTAGTCAGTTTCAGTTTTATGGGCTTCATCACAAGTT
TACATATGGACTTAGGCTCGATTCCACCCGCCTAGGTTGGATGGGCGTGCTTCGTTGGAA
AGCATGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGGA
CATCTCGGTATTTCGTGCGCAAGACGACTATACCGCCACCCAAACGGCCTAGGAGAATCC
ATGTCAGCCCATGCCATGACACGCCCATTCTCTTCGGCGTAAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 28-K113192-0022-088-G05C-8409
GenBank Accession LN498924 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K113192-0022-088-G05C-8409
ATTTACGCCGAAGAGAATGGGCGTGTCATGGCATGGGCTGACATGGATTCTCCTAGGCCG
TTTGGGTGGCGGTATAGTCGTCTTGCGCACGAAATACCGAGATGTCCCCATGGGCGACAC
TCACACCTCGCTCACACTCGGGACAGCCTAGGCGGATCCATGCTTTCCAACGAAGCACGC
CCATCCAACCTAGGCGGGTGGAATCGAGCCTAAGTCCATATGTAAACTTGTTGATGAAGC
CCATTAAAACTGAAACTTGACTAATTCGGGTCGGACTCAAATCAAAAACATACAGTATGT
TGAAAAAGATGAAGTGAAGACAATGGTGGGTTCCTA
Primers used for PCR 43lf - 8474, show primer details
   
Sequence Name 73-K113840-0022-088-G05C-43lf
GenBank Accession LN512941 [GenBank]
Sequencing primer gene specific primer, 43lf: 5'-GCTTAACCTGCACTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113840-0022-088-G05C-43lf
TAAATATTTTGTATAGATTTGAGATGTTTTGTTTCTCACTTTGAATGAAAAAAAAAAAAC
TTTTACAAAAAAAATTGTTGTCGGTTTATGTATAAAAAATTAAAGTTGAAAATTGAACCA
AAATAAACCTACTTTATGGATTATAGGAACCAACCATTGTCTTCACTTCATCTTTTTCAA
CAAACGGAAGGTTTTTGATTTGAGTCCAACCCAAATTATTCAAGTTTCATTTTTAAGGGG
CTTCATCAACAATTTTACATATGGACTTAGGCTCAATTCCCCCCCCCAAGGTGGAAGGGG
CGTGCTTCTTTGAAAAGCAGGGATCCCCCAAGGCTGTCCCAAGTGTAACCAAGGTGTAAT
TGTCCCCCATGGGGACATCTCGGTATTTCGGGCGCAAAACAACAATCCCGCCCCCCAAAC
GGCCAAGGAAAATCCATGTCACCCCATGCCAGGACACCCCCATTCTCTTCGGCGTAAAAG
GGCTTCATGTCCGGAAAATCTACTTGGATCACCATTGAGTATAAGGGTCAATATGGAAAA
AAAAAAAAAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTCC
   
Sequence Name 65-K113841-0022-088-G05C-8409
GenBank Accession LN511444 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K113841-0022-088-G05C-8409
ATTTACGCCGAAGAGAATGGGCGTGTCATGGCATGGGCTGACATGGATTCTCCTAGGCCG
TTTGGGTGGCGGTATAGTCGTCTTGCGCACGAAATACCGAGATGTCCCCATGGGCGACAC
TCACACCTCGCTCACACTCGGGACAGCCTAGGCGGATCCATGCTTTCCAACGAAGCACGC
CCATCCAACCTAGGCGGGTGGAATCGAGCCTAAGTCCATATGTAAACTTGTTGATGAAGC
CCATTAAAACTGAAACTTGACTAATTCGGGTCGGACTCAAATCAAAAACATACAGTATGT
TGAAAAAGATGAAGTGAAGACAATGGTTGGTTCCTATAATCCATAACGTAGGTTTATCTT
GGTTCAATTTTCAACTTTAATTTCTTATACATAAACCGACAACAATTTTTTTAGTAAAAG
TTTTTTTTTTTTCTTTCAAAGTGAAAAACAAAACATCTCAAATCTTTAACAAAATATTTT
TATATCATTTTTAAACTTTGGCTCAGGCATAGGGAGGGTAAA

Last Updated on 06.12.2018 16:11