Sequences from confirmation amplicon


Confirmation sequences for line 398H12 and hit At5g11480 are shown.
Line ID 398H12
Locus At5g11480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3671161
Primers used for PCR CD82 - 8474, show primer details
   
Sequence Name 35-K105566-0022-398-H12C-CD82
GenBank Accession LN501833 [GenBank]
Sequencing primer gene specific primer, CD82: 5'-CAACAATACCCCATCTTCAAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K105566-0022-398-H12C-CD82
GCAATCTGTTCATCTAACCAGTGGGGATAGTCTATTGGTTTTGTCCTATTGACTATTGAC
TATTGATTGAGACTAGAGACTGATTCCCACTCAATGCCTCACTTGGCCACTACAGAATTC
AAATGCTAGGCGTTAGCCCATGATTAGTGTGGACTCTAGCAAAACCCAGTTATAGAAGAA
TCATTTCAATAAATCAATTGACCTAAGCTTCCAACAACAACAAAAACTGACTTCTCCTTA
CTGCTTTACCAACACTCTCCAATCTCCTCATGAACATTAAAAAACTGACTACATCACTCT
GTCTCAAAGATGTGATTGCTTCAGTTTCACATGACATTTCAACGGGAATAAGCCAAGTTG
TTCTCTTAATCTATCATCTACTTAACCAGTGCTTCAGGTCCCCAAAATGGAAGCTTTTGA
TCCATAAATATAATGACACTCAAAATAATGTAGAAATCACACACCAGCTAAATGATAAGC
ACGATGGAGCAAAACTCAGCTTACCAGGTTTCTTAGAAGTCAAGGCAAGGCGTTTCCTCC
TCACCAACGAATTGAGAAGCGATGATTTCCCAACATTGGATCTCCCAACAAGCGCAAACT
CCGGCAACCCATCGGCCGGACAATCCTCCGTCTTGACACTACTCTACATGTATATATTCA
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 67-K105567w-0022-398-H12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K105567w-0022-398-H12C-8409
GCCATTTACATTGAATATTGAATATATACATGTAGAGTAGTGTCAAGACGGAGGATTGTC
CGGCCGATGGGTTGCCGGAGTTTGCGCTTGTTGGGAGATCCAATGTTGGGAAATCATCGC
TTCTCAATTCGTTGGTGAGGAGGAAACGCCTTGCCTTGACTTCTAAGAAACCTGGTAAGC
TGAGTTTTGCTCCATCGTGCTTATCATTTAGCTGGTGTGTGATTTCTACATTATTTTGAG
TGTCATTATATTTATGGATCAAAAGCTTCCATTTTGGGGACCTGAAGCACTGGTTAAGTA
GATGATAGATTAAGAGAACAACTTGGCTTATTCCCGTTGAAATGTCATGTGAAACTGAAG
CAATCACATCTTTGAGACAGAGTGATGTAGTCAGTTTTTTAATGTTCATGAGGAGATTGG
AGAGTGTTGGTAAAGCAGTAAGGAGAAGTCAGTTTTTGTTGTTGTTGGAAGCTTAGGTCA
ATTGATTTATTGAAATGATTCTTCTATAACTGGGTTTTGCTAGAGTCCACACTAATCATG
GGCTAACGCCTAGCATTTGAATTCTGTAGTGGCCAAGTGAGGCATTGAGTGGGAATCAGT
CTCTAGTCTCAATCAATAGTCAATAGTCAATAGGACAAAACCAATAGACTATCCCCACTT
GGTTAGATTGAACAGATTTGCCTTGGAGCATCGGCATTTGTTGGTTTCTAGTACTTTTGA
AG
Primers used for PCR 49wp - 8474, show primer details
   
Sequence Name 33-K118837-0022-398-H12C-49wp
GenBank Accession LN501196 [GenBank]
Sequencing primer gene specific primer, 49wp: 5'-GAGAGAGATTTTGGTTCACTGGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118837-0022-398-H12C-49wp
CCACGACCCGATTTTGCTATATACTCCTTATACTTTTTGTTTTCTTTCTAATTTACCCAT
TTACTCTCTAATTAGAAGATAGTGGCGAAATGATACAATCGTTAAAATAGAGCGGGCAAA
TTGTAATATAAGGAAAGTTCATCGTCCAGATTACCACCATCAAAACGTAGCGTTTTGATG
TGGATATTGTTTCCCCGACCTCCTCCGCATCCGTAAGTTCAGTCCTACATCCATGGCTGC
TCACAGAATCTAAAGAGAACTAAAAAGCTCATAAGACTATTACTTAGAAGAGAATCGATC
AGATATGATTTTAGCGCATCTTCCAAGATTCCATCTCTCAATCCTCGCGAAACCTTCTTT
CTCTCTCTCTTCTTCGCATTTCAGTTTCTTAAACCCTAAGCCTTCGGTAACAGTATCTCG
AACCCTTTTCTCATTCGCGTCTAAATCGAATCTCGCCACTGTTGAACCCATACCATTATC
GGTCTCAGATTCAACCGATTTGGATGATGCACCTGTCGAGATTGCGCTCGACAAGCTTTT
TATTCCGCCGGAGACCGATATATCCGGTGAAGACTCGGCGAGTTTAACGACGAGGATACT
TAAGGGTTCGAATATTGTGCTGAGCAAGTACGCGAGTCCACAATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCATAAATG
TAGATGTCCGC
   
Sequence Name 09-K118836-0022-398-H12C-8409
GenBank Accession LN490414 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118836-0022-398-H12C-8409
ACATATTCGACCCTTAAGTATCCTCGTCGTTAACTCGCCGAGTCTTCACCGGATATATCG
GTCTCCGGCGGAATAAAAAGCTTGTCGAGCGCAATCTCGACAGGTGCATCATCCAAATCG
GTTGAATCTGAGACCGATAATGGTATGGGTTCAACAGTGGCGAGATTCGATTTAGACGCG
AATGAGAAAAGGGTTCGAGATACTGTTACCGAAGGCTTAGGGTTTAAGAAACTGAAATGC
GAAGAAGAGAGAGAGAAAGAAGGTTTCGCGAGGATTGAGAGATGGAATCTTGGAAGATGC
GCTAAAATCATATCTGATCGATTCTCTTCTAAGTAATAGTCTTATGAGCTTTTTAGTTCT
CTTTAGATTCTGTGAGCAGCCATGGATGTAGGACTGAACTTACGGATGCGGAGGAGGTCG
GGGAAACAATATCCACATCAAAACGCTACGTTTTGATGGTGGTAATCTGGACGATGAACT
TTCCTTATATTACAATTTGCCCGCTCTATTTTAACGATTGTATCATTTCGCCACTATCTT
CTAATTAGAGAGTAAATGGGTAAATTAGAAAGAAAACAAAAAGTATAAGGAGTATATAGC
AAAATCGGGTCGTTGGCATCTCCTTCCACCTCTCCAAGCACGGACGAGACTTCGACCAGT
GAATCCAAATCTCTCT
Primers used for PCR CD83 - 8474, show primer details
   
Sequence Name 34-K118837-0022-398-H12C-CD83
GenBank Accession LN501609 [GenBank]
Sequencing primer gene specific primer, CD83: 5'-CTTCGCATTTCAGTTTCTTAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118837-0022-398-H12C-CD83
CGACCTTTTCTCATTCGCGTCTAATCGAATCTCGCCACTGTTGAACCCATACCATTATCG
GTCTCAGATTCAACCGATTTGGATGATGCACCTGTCGAGATTGCGCTCGACAAGCTTTTT
ATTCCGCCGGAGACCGATATATCCGGTGAAGACTCGGCGAGTTTAACGACGAGGATACTT
AAGGGTTCGAATATTGTGCTGAGCAAGTACGCGAGTCCACAATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGACGTTATTAT
   
Sequence Name 10-K118836-0022-398-H12C-oriL
GenBank Accession LN490885 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118836-0022-398-H12C-oriL
ATATTCGACCCTTAGTATCCTCGTCGTTAAACTCGCCGAGTCTTCACCGGATATATCGGT
CTCCGGCGGAATAAAAAGCTTGTCGAGCGCAATCTCGACAGGTGCATCATCCAAATCGGT
TGAATCTGAGACCGATAATGGTATGGGTTCAACAGTGGCGAGATTCGATTTAGACGCGAA
TGAGAAAAGGGTTCGAGATACTGTTACCGAAGGCTTAGGGTTTAAGAAACTGANAATGCG
AAG

Last Updated on 06.12.2018 16:11