Sequences from confirmation amplicon


Confirmation sequences for line 088G09 and hit At5g12930 are shown.
Line ID 088G09
Locus At5g12930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4083589
Primers used for PCR 76zk - 8474, show primer details
   
Sequence Name 47-K129065-0022-088-G09C-76zk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 76zk: 5'-CTAACAAAACTGCAGCCTCCTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K129065-0022-088-G09C-76zk
TCTTGATCAGTCATCCTCACTTTATGGCCAGTTTCCCCGCTTTCACGGGGATGATACAAC
CTGGTAAGATCTAACTTTTAGCAAGCAATACCCAAGTTAAGTGGAAAAGCCGGACACTGA
CACATTACAACGTGGGAGCAGGAGTGTCTTTGTGAGAACGCATGGAACTGGTTTTCAATC
TCTTTTACCTCTTTTTTATGCTTAAACATGACTAAATTGACTTAAGTTTTCTTATACTCA
TGATGTCATGTTTAAGCGCTTTTAGTTTACTTAAATCAGGAACAAATCTTTTAAGCATTA
CATGAACTCTTGCATTGAGAGTGTCAACCATATTGAGATAGAAAGACAGGTTTGTGTTTG
CCTTGTGGTCCTTTCTTCTGTCGGCTTTTAATTACCTCGGAAACATTATTATTAGTTTAT
TACATTAGCATAAAAGAAACACTCAGTTTAGTGAGAGCTATTGATATGATGAAGTCAACA
CAAATAACTTTTTTTATTATATTATACTCTTTAGCTACAATATATAACTCGAGATTCACC
AACTTCACATCTGGCAGCTGGTTCTGCACTTCAGACATATATTAAACAGGTCCAGTCATA
ACTTACTCAAACAATTTTAAATTTGAACTTAACAAACTAACTATGGATCTCTTGTCTAAA
CTTTTAAGTACAATTCAATGGTAATTTTTGTTTTTTTCTCAGAGCTTGAACTTATCAGCA
GACCAATACTTATTAAACCCATCTCTTATGTGAACCAGAGGAATCTCTTGGGATGGTCTG
AATTGGCCTTCTAGGTTTCTTTGAATCACCTGTAGAGGAACCCCGTCTCACCCTTGAAGA
AGAAGAAACTGAAACAGGCTTCCGAGTAGTCGATGGACTAACTACTGAAGGCGGCTTTAC
AGATTTCACAAGTGACGTCGAGAATTTAGCTGGCGCTGAATTATTGGTTTTGTCCAAAGT
TGCGGGTTCCTCCTTTACGGAATCAACTGG
   
Sequence Name 05-K129064-0022-088-G09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K129064-0022-088-G09C-8409
GCCATTTCATTGAATATATCCGGAAGTACAGGTCACACAGCTCACTAGGCTCCGGAGGTC
CTCCTATTCACCTGCAGTCTCAAATACCCATGAGTTCCCAATGAGAATGGATAATCTTCT
TTATGATATGGATGTTCTTACAGCTTCTTCATCCTCTGATTCAGAAGCTACTGTAAACAC
ACCCCAGCGAGCTGTGCTGGAGGCTCCAGTTGATTCCGTAAAGGAGGAACCCGCAACTTT
GGGACAAACCAATAAATCAGCGCCAGCTAAATCCTCGACGTCACTTGTGAAATCTGTAAA
GCCGCCTTCAGTAGTTAGTCCATCGACTACTCGGAAGCCTGTTTCAGTTTCTTCTTCTTC
AAGGGTGAGACGGGGTTCCTCTACAGGTGATTCAAAGAAACCTAGAAGGCCAATTCAGAC
CATCCCAAGAGATTCCTCTGGTTCACATAAGAGATGGGTTTAATAAGTATTGGTCTGCTG
ATAAGTTCAAGCTCTGAGAAAAAAACAAAAATTACCATTGAATTGTACTTAAAAGTTTAG
ACAAGAGATCCATAGTTAGTTTGTTAAGTTCAAATTTAAAATTGTTTGAGTAAGTTATGA
CTGGACCTGTTTAATATATGTCTGAAGTGCAGAACCAGCTGCCAGATGTGAAGTTGGTGA
ATCTCGAGTTATATATTGTAGCTAAAGAGTATAATATAATAAAAAAAGTTATTTGTGTTG
ACTTCATCATATCAATAGCTCTCACTAAACTGAGTGTTTCTTTTATGCTAATGTAATAAA
CTAATAATAATGTTTCCGAGGTAATTAAAAGCCGACAGAAGAAAGGACCACAAGGCAAAC
ACAAACCTGTCTTTCTATCTCAATATGGTTGACACTCTCAATGCAAGAGTTCATGTAATG
CTTAAAAGATTTGTTCCTGATTTAAGTAAACTAAAAACGCTTAAACATGACATCATG

Last Updated on 06.12.2018 16:11